Rabbit anti-Human Ki-67 Monoclonal Antibody | anti-Ki-67 antibody

Anti - Ki-67

Cat. Num. AAA684060

• Gene Names: MKI67; KIA; MIB-1
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: Ki-67; Monoclonal Antibody; Anti - Ki-67; anti-Ki-67 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: C16-I
• Specificity: Human
• Form/Format: 20 mM Tris-HCI, pH 8.0
• Sequence Length: 3256

• Applicable Applications for anti-Ki-67 antibody: Immunohistochemistry (IHC) - Formalin/Paraffin
• Application Notes: IHC-P dilution 1:100 - 1:200; IHC-Fr application and dilution to be tested by the user.; ; Precautions:; 1. We strongly recommend to use Primary Antibody Diluent, eventually alternative diluent (containing protease free BSA at the concentrations >1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.; 2. Centrifuge the vial before use.; 3. Intended for professional test in laboratories.; 4. Do not use after expiration date stamped on vial label.; 5. Avoid contamination of the reagent.; 6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.; 7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.; 8. Disposal of waste material must be conducted in accordance with local regulations.; 9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
• Immunogen: Peptide derived from C-terminal region of human Ki-67. Antibody recognizes the epitope between Lys3243 - Asp3255.
• Stabilizer: 20 mg/ml BSA
• Preservative: 0.05% NaN3
• Cellular Localization: Nucleus
• Positive Control: Tonsil
• IHC-P Protocol-Instruction Manual: 1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.; 2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.; 3. Rinse in distilled water.; 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.; 5. Wash in distilled water.; 6. For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, 0.05% Tween- 20*, and incubate in water bath at 96-98ºC for 20-25 minutes. (Alternatively adjust to your own protocol, keeping the required pH); 7. Transfer the slide to room temperature and let it cool down (in Tris-EDTA buffer, pH 9.0) for 15 minutes.; 8. Rinse in distilled water.; 9. Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.; 10. CONCENTRATED:; Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.; READY TO USE (RTU):; Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.; 11. Wash twice 5 minutes with buffer A.; 12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested.; 13. Wash twice 5 minutes with buffer A.; 14. Apply the chromogen (DAB), 1 - 3 minutes.; 15. Wash in water – 10 minutes.; 16. Stain in hematoxylin for 5 minutes.; 17. Wash in water – 10 minutes.; 18. Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.; 19. Wash the section in 2 changes of xylene for 2 minutes each.; 20. Mount the slide for observation.; ; *Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):; Tris ------------- 1.21 g; EDTA ------------ 0.37 g; Distilled water ----------- 1000 ml Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water.
• Preparation and Storage: Store at +4°C.

Immunohistochemistry (IHC)

(AHigh Grade SIL (squamous intraepithelial lesion) of the uterine cervix showing full-thickness Ki-67 immunoexpression in the dysplastic epithelium (A), B-cell lymphoma showing high proliferating index visualised by the Ki-67 immunohistochemistry (B) and proliferating germinal centre cells of the lymphoid follicle visualised by the Ki-67 immunohistochemistry (C). All, formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Ki-67 (MBS684060) monospecific clonal antibody according to related datasheet.)

Immunohistochemistry (IHC)

(BHigh Grade SIL (squamous intraepithelial lesion) of the uterine cervix showing full-thickness Ki-67 immunoexpression in the dysplastic epithelium (A), B-cell lymphoma showing high proliferating index visualised by the Ki-67 immunohistochemistry (B) and proliferating germinal centre cells of the lymphoid follicle visualised by the Ki-67 immunohistochemistry (C). All, formalin fixed, paraffin embedded human tissues (4um sections) stained with anti - Ki-67 (MBS684060) monospecific clonal antibody according to related datasheet.)

Immunohistochemistry (IHC)

(CHigh Grade SIL (squamous intraepithelial lesion) of the uterine cervix showing full-thickness Ki-67 immunoexpression in the dysplastic epithelium (A), B-cell lymphoma showing high proliferating index visualised by the Ki-67 immunohistochemistry (B) and proliferating germinal centre cells of the lymphoid follicle visualised by the Ki-67 immunohistochemistry (C). All, formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Ki-67 (MBS684060) monospecific clonal antibody according to related datasheet.)

NCBI and Uniprot Product Information

• NCBI GI #: 118572663
• NCBI GeneID: 4288
• UniProt Accession #: P46013; Q5VWH2
• NCBI Official Full Name: Antigen KI-67
• NCBI Official Synonym Full Names: marker of proliferation Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA; MIB-1
• NCBI Protein Information: antigen KI-67; proliferation-related Ki-67 antigen; antigen identified by monoclonal antibody Ki-67
• UniProt Protein Name: Antigen KI-67
• UniProt Gene Name: MKI67
• UniProt Entry Name: KI67_HUMAN

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

Function: Thought to be required for maintaining cell proliferation.

Subunit structure: Interacts with KIF15. Binds through the FHA domain to NIFK. Part of a complex composed at least of ASCL2, C11orf30/EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity. Ref.4 Ref.5 Ref.13 Ref.19

Subcellular location: Nucleus. Nucleus › nucleolus. Chromosome. Note: Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. Ref.20

Developmental stage: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Sequence similarities: Contains 1 FHA domain.