Immunohistochemistry (IHC)
(Poorly differntiated human neuroblastoma tissue stained with anti-CD57 monospecific clonal antibody. Formalin fixed, paraffin embedded human tissue (4um section) stained according to related datasheet.)
Rabbit CD57 Monoclonal Antibody | anti-CD57 antibody
Anti-CD57
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval immerse the slide in citrate buffer, pH 6.0*, and incubate in water bath for 25-35 minutes at 96-98?C.
7. Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (buffer A), 2 x 5 minutes
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested (Please Inquire).
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1-3 minutes.
15. Wash in distilled water, 2 x 5 minutes.
16. Rinse in CuSO4.5H2O solution /0,90g NaCl + 0,50g CuSO4.5 H2O in 100ml
distilled water/
17. Wash in distilled water, 1 x 2 minutes.
18. Stain in hematoxylin for 5 minutes.
19. Wash in distilled water, 3 x 2 minutes.
20. Rinse in 37mM ammonium hydroxide solution.
21. Wash in distilled water, 1 x 2 minutes.
22. Mount the slide for observation.
* Citrate Buffer (10mM Citric Acid, 0.05% Tween-20, pH 6.0):
Citric acid (anhydrous) ------------- 1.92 g; Distilled water ------------ 1000 ml
Mix to dissolve in 700 ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water.
Store this solution at room temperature for 3 months or at 4ºC for longer storage.
Immunohistochemistry (IHC)
(Poorly differntiated human neuroblastoma tissue stained with anti-CD57 monospecific clonal antibody. Formalin fixed, paraffin embedded human tissue (4um section) stained according to related datasheet.)
Immunohistochemistry (IHC)
(Poorly differntiated human neuroblastoma tissue stained with anti-CD57 monospecific clonal antibody. Formalin fixed, paraffin embedded human tissue (4um section) stained according to related datasheet.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
B3GAT1: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Belongs to the glycosyltransferase 43 family.
Protein type: Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; Transferase; Glycan Metabolism - heparan sulfate biosynthesis; EC 2.4.1.135
Chromosomal Location of Human Ortholog: 11q25
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity; metal ion binding; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity
Biological Process: carbohydrate metabolic process; chondroitin sulfate metabolic process; chondroitin sulfate proteoglycan biosynthetic process; glycosaminoglycan metabolic process; protein amino acid glycosylation
Research Articles on CD57
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Product Notes
The CD57 b3gat1 (Catalog #AAA684292) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD57 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD57 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry -Paraffin (IHC-P). Immunohistochemistry (IHC-P) Paraffin: 1:100 - 1:200. Researchers should empirically determine the suitability of the CD57 b3gat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD57, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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