Immunohistochemistry (IHC)
Rabbit Human IgG Antibody | anti-IGHG1 antibody
Anti - Human IgG
Applications
Immunohistochemistry
Synonyms
Human IgG; Anti - Human IgG; anti-IGHG1 antibody
Host
Rabbit
Clone Number
E20-V
Specificity
Human
Applicable Applications for anti-IGHG1 antibody
Immunohistochemistry (IHC) - Paraffin
Application Notes
Dilution: 1:100-1:300
IHC-P Protocol - Instruction Manual:
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:300 for 1 hour in the closed wet chamber.
READY TO USE (RTU): Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB).
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well.
Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage.
Ventana Protocol - Instruction Manual:
SHORT APPLICATION PROTOCOL FOR VENTANA BENCHMARK SLIDE STAINING SYSTEM
1. Drying (Enter).
2. Heating glass (72°C), incubation 4 min. Drying.
3. Deparafinization (Enter).
4. Heating (72°C) with the medium temperatures. Deparafinization.
5. Cell conditioning (Enter).
6. ULTRA Conditioner #1 (Enter).
7. Heating glass (95°C), incubation 8 min (Cell conditioner #1).
8. ULTRA CC1 solution application – 20 min (Enter).
9. Titration (Enter).
10. Hand apply – primary antibody. Incubation 24 min.
11. UltraWash (Enter).
12. Nuclear stain (Enter).
13. Hematoxylin application – one drop (nuclear stain), cover and incubate 8 min.
14. After nuclear stain (Enter).
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.
IHC-P Protocol - Instruction Manual:
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:300 for 1 hour in the closed wet chamber.
READY TO USE (RTU): Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB).
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well.
Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage.
Ventana Protocol - Instruction Manual:
SHORT APPLICATION PROTOCOL FOR VENTANA BENCHMARK SLIDE STAINING SYSTEM
1. Drying (Enter).
2. Heating glass (72°C), incubation 4 min. Drying.
3. Deparafinization (Enter).
4. Heating (72°C) with the medium temperatures. Deparafinization.
5. Cell conditioning (Enter).
6. ULTRA Conditioner #1 (Enter).
7. Heating glass (95°C), incubation 8 min (Cell conditioner #1).
8. ULTRA CC1 solution application – 20 min (Enter).
9. Titration (Enter).
10. Hand apply – primary antibody. Incubation 24 min.
11. UltraWash (Enter).
12. Nuclear stain (Enter).
13. Hematoxylin application – one drop (nuclear stain), cover and incubate 8 min.
14. After nuclear stain (Enter).
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.
Buffer
20 mM Tris-HCl, pH 8.0
Stabilizer
20 mg/ml BSA
Preservative
0.05% NaN3
Immunogen
Peptide derived from internal domain of human IgG-1 chain C region. Antibody recognizes the epitope between Val167 - Val185.
Cellular Localizaton
Secreted
Positive Control
Human Tonsil Tissue
Precautions
1. We strongly recommend to use a Primary Antibody Diluent, eventually alternative diluent (containing protease free BSA at the concentrations > 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.
2. Centrifuge the vial before use.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
2. Centrifuge the vial before use.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Preparation and Storage
Store at +4°C.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Ig gamma-1 chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Official Symbol
IGHG1
UniProt Protein Name
Ig gamma-1 chain C region
Protein Family
UniProt Gene Name
IGHG1
UniProt Entry Name
IGHG1_HUMAN
Uniprot Description
Subcellular location: Secreted.
Involvement in disease: Multiple myeloma (MM) [MIM:254500]: A malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia.Note: The disease is caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving IGHG1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.
Miscellaneous: Nie has the G1M17 allotypic marker, 97-K, and the G1M1 markers, 239-D and 241-L. KOL and EU sequences have the G1M3 marker and the G1M (non-1) markers.
Sequence caution: The sequence AAC82527.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on IGHG1
Similar Products
Product Notes
The IGHG1 ighg1 (Catalog #AAA684242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Human IgG can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) - Paraffin. Dilution: 1:100-1:300 IHC-P Protocol - Instruction Manual: 1. Deparaffinize the section in 3 changes of xylene, 10 minutes each. 2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each. 3. Rinse in distilled water, 2 x 5 minutes. 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes. 5. Wash in distilled water, 2 x 5 minutes. 6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes. 7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes. 8. Rinse in distilled water, 2 x 5 minutes. 9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes. 10. CONCENTRATED: Incubate the section with primary antibody at the dilution 1:100 - 1:300 for 1 hour in the closed wet chamber. READY TO USE (RTU): Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber. 11. Wash 3 x 5 minutes with buffer A. 12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). 13. Wash 3 x 5 minutes with buffer A. 14. Apply the chromogen (DAB), 1 - 3 minutes. 15. Wash in water, 2 x 5 minutes. 16. Stain in hematoxylin for 5 minutes. 17. Wash in distilled water, 3 x 2 minutes. 18. Mount the slide for observation. * Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0): Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage. Ventana Protocol - Instruction Manual: SHORT APPLICATION PROTOCOL FOR VENTANA BENCHMARK SLIDE STAINING SYSTEM 1. Drying (Enter). 2. Heating glass (72°C), incubation 4 min. Drying. 3. Deparafinization (Enter). 4. Heating (72°C) with the medium temperatures. Deparafinization. 5. Cell conditioning (Enter). 6. ULTRA Conditioner #1 (Enter). 7. Heating glass (95°C), incubation 8 min (Cell conditioner #1). 8. ULTRA CC1 solution application – 20 min (Enter). 9. Titration (Enter). 10. Hand apply – primary antibody. Incubation 24 min. 11. UltraWash (Enter). 12. Nuclear stain (Enter). 13. Hematoxylin application – one drop (nuclear stain), cover and incubate 8 min. 14. After nuclear stain (Enter). 15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min. Researchers should empirically determine the suitability of the IGHG1 ighg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Human IgG, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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