Rabbit anti-Human CD8 Monoclonal Antibody | anti-CD8 antibody

Anti - CD8

Cat. Num. AAA684124

• Gene Names: CD8A; CD8; MAL; p32; Leu2
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: CD8; Monoclonal Antibody; Anti - CD8; anti-CD8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: P17-V
• Specificity: Human
• Sequence Length: 235

• Applicable Applications for anti-CD8 antibody: Immunohistochemistry (IHC) - Formalin/Paraffin
• Buffer: 20 mM Tris-HCI, pH 8.0
• Stabilizer: 20 mg/ml BSA
• Preservative: 0.05% NaN3
• Immunogen: Peptide derived from C-terminal region of human CD8. Antibody recognizes the epitope between Pro218 - Tyr234 of CD8-alpha human protein.
• Positive control: human tonsil tissue
• Cellular localization: membrane
• IHC-P Protocol–Istruction Manual: 1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.; 2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.; 3. Rinse in distilled water.; 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.; 5. Wash in distilled water.; 6. For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, 0.05% Tween-20*, and incubatein water bath at 96-98 degree C for 20-25 minutes.; 7. Transfer the slide to room temperature and let it cool down for 15 minutes in the buffer used in the step #6; 8.Rinse in distilled water.; 9. Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.; 10. Ready to use (RTU): Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.; 11.Wash twice 5 minutes with buffer A.; 12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard mmunohistochemistry protocol.; 13.Wash twice 5 minutes with buffer A.; 14. Apply the chromogen (DAB), 1 -3 minutes.; 15. Wash in water –10 minutes.; 16. Stain in hematoxylin for 5 minutes.; 17.Wash in water–10 minutes.8.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.; 19. Wash the section in 2 changes of xylene for 2 minutes each.; 20.Mount the slide for observation.
• Precautions: 1. We strongly recommend to use DB Primary Antibody Diluent eventually alternative diluent (containing protease free BSA at the concentrations 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be oided.; 2.Centrifuge the vial before use.3.Intended for professional use in laboratories.; 4.Do not use after expiration date stamped on vial label.; 5.Avoid contamination of the reagent.; 6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.; 7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.; 8. Disposal of waste material must be conducted in accordance with local regulations.; 9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
• Preparation and Storage: Store at +4°C. Do not freeze!

Immunohistochemistry (IHC)

(CD8 expression in T-lymphocytes of the palatine tonsil. Formalin fixed, paraffin embedded human tissue (4 ?msection) stained with anti -CD8 (MBS684124) monospecific clonal antibody)

NCBI and Uniprot Product Information

• NCBI GI #: 116035
• NCBI GeneID: 925
• UniProt Accession #: P01732; Q13970; Q4ZG17; D6W5M8
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; MAL; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: CD81 antigen
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not

By similarity.

Involvement in disease: CD8 deficiency, familial (CD8 deficiency) [MIM:608957]: An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.