Rabbit anti-Human EBV/LMP-1 Monoclonal Antibody | anti-EBV/LMP-1 antibody

Anti - EBV/LMP-1

Cat. Num. AAA684063

• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: EBV/LMP-1; Monoclonal Antibody; Anti - EBV/LMP-1; anti-EBV/LMP-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: S20-D
• Specificity: Human
• Sequence Length: 386

• Applicable Applications for anti-EBV/LMP-1 antibody: Immunohistochemistry (IHC) - Formalin/Paraffin
• Application Notes: IHC-P; Dilution: 1:100-1:200
• Immunogen: Peptide derived from the C-terminal region of Epstein Barr virus (EBV), Latent Membrane Protein-1 (LMP -1). Antibody recognizes the epitope between Ser369 -Tyr384.
• Buffer: 20 mM Tris-HCI, pH 8.0
• Stabilizer: 20 mg/ml BSA
• Preservative: 0.05% NaN3
• Immunogen: Peptide derived from the C-terminal region of Epstein-Barr virus (EBV), Latent Membrane Protein-1 (LMP-1). Antibody recognizes the epitope between Ser369 - Tyr384.
• Cellular localization: nucleus
• Positive control: lymph node Hodgkin’s lymphoma tissue
• IHC-P PROTOCOL: 1.Deparaffinize the section in 3 changes of xylene, 5 minutes each.; 2.Wash the section in 96%, 80% and 0% thyl alcohol for 10 minutes each. .; 3.Rinse in distilled water. .; 4.Block the endogenous peroxidase by incubating the tissuein 3% hydrogen peroxide (H2O2) for 10 minutes. .; 5.Wash in distilled water for 5 minutes. .; 6.For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in ater bath at 96°C for 40 minutes. (Alternatively adjust to your own protocol, keeping the required pH) .; 7.Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes. .; 8.Rinse in distilled water. .; 9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.; 10.CONCENTRATED:Incubate the section with primary antibody at the dilution 1:100 -1:200 for 1 hour in the closed wet chamber. .; .READY TO USE (RTU):Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.; 11.Wash twice 5 minutes with buffer A. .; 12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP -Peroxide -DAB). Micropolymer-HRP detection kit rabbit/mouse .; 13.Wash twice 5 minutes with buffer A. .; 14.Apply the chromogen (DAB), 1 -3 minutes. .; 15.Wash in water for 10 minutes. .; 16.Stain in hematoxylin for 5 minutes. .; 17.Wash in water for 10 minutes. .; 18.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.; 19.Wash the section in 2 changes of xylene for 2 minutes each. .; 20.Mount the slide for observation.; ; ; Citrate Buffer(10mM Citric Acid, 0.05% Tween-20, pH 6.0):; Citric acid (anhydrous) ----------1.92 g; Distilled water ---------1000 ml.; Mix to dissolve in 700 ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5 ml of Tween-20 and mix well. ; Adjust the final volume to 1 liter with distilled water.; ; Store this solution at room temperature for 3 months or at +4°C for longer storage.
• PRECAUTIONS: 1.-We strongly recommend to use Primary Antibody Diluent eventually alternative diluent containing protease free BSA at the concentrations ? 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.; 2.Centrifuge the vial before use.; 3.Intended for professional use in laboratories. .; 4.Do not use after expiration date stamped on vial label. .; 5.Avoid contamination of the reagent. .; 6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results. .; 7.The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous. .; 8.Disposal of waste material must be conducted in accordance with local regulations. .; 9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
• Preparation and Storage: Store at +4°C.

Test Data

(HRS cells of the classical Hodgkin Lymphoma showing cytoplasmic expression of the EBV LMP-1 protein. Formalin fixed, paraffin embedded human tissue (4 Um section) stained with anti - EBV LMP-1 (MBS684063) monospecific clonal antibody according to related DB Biotech datasheet.)

NCBI and Uniprot Product Information

• NCBI GI #: 126373
• NCBI GeneID: 3783750
• UniProt Accession #: P03230; Q777A4; 1011284A
• NCBI Official Full Name: Latent membrane protein 1
• NCBI Official Symbol: LMP1
• NCBI Protein Information: LMP1; hypothetical protein
• UniProt Protein Name: Latent membrane protein 1
• UniProt Gene Name: LMP1
• UniProt Synonym Gene Names: LMP-1
• UniProt Entry Name: LMP1_EBVB9

Uniprot Description

Function: Acts as a CD40 functional homolog to prevent apoptosis of infected B-lymphocytes and drive their proliferation. Functions as a constitutively active tumor necrosis factor receptor that induces the activation of several signaling pathways, including those of the nuclear factor-kappa-B family. LMP1 signaling leads to up-regulation of antiapoptotic proteins and provide growth signals in latently infected cells. It is a short-lived protein probably degraded by the proteasome.

Subunit structure: Interacts (via PXQXT motif) with host tumor necrosis factor receptor-associated factor (TRAF) proteins TRAF1, TRAF2, TRAF3 and TRAF5.

Subcellular location: Host cell membrane; Multi-pass membrane protein Ref.8.

Domain: Two regions, C-terminal-activating region 1 (CTAR1) and CTAR2, have been identified within the cytoplasmic carboxy terminal domain that activates NF-kappa-B. Ref.7

Post-translational modification: Ubiquitinated on the N-terminus. Ref.8

Sequence similarities: Belongs to the herpesviridae LMP-1 family.