MIF recombinant protein

MIF

Cat. Num. AAA517294

• Gene Names: MIF; GIF; GLIF; MMIF
• Applications: Cell Assay
• Synonyms: MIF; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; EC=5.3.3.12; Phenylpyruvate tautomerase; GLIF; MMIF; Recombinant Human Macrophage Migration Inhibitory Factor (MIF); MIF recombinant protein

• Host: E Coli
• Form/Format: Recombinant human MIF was lyophilized from a 0.2 um filtered solution PBS pH7.4

• Applicable Applications for MIF recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: The activity was determined by the ability to bind recombinant human CD74 in functional ELISA.)

SDS-Page

(Purity: Recombinant Human MIF resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for MIF recombinant protein

Recombinant Human MIF was expressed in E Coli cells. The protein accession number is P14174
Macrophage Migration Inhibitory Factor (MIF) is a proinflammatory cytokine. MIF is involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Product Categories/Family for MIF recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4505185
• NCBI GeneID: 4282
• NCBI Accession #: NP_002406.1
• NCBI GenBank Nucleotide #: NM_002415.1
• UniProt Accession #: P14174; Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3
• Molecular Weight: 12,476 Da
• NCBI Official Full Name: macrophage migration inhibitory factor
• NCBI Official Synonym Full Names: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• NCBI Official Symbol: MIF
• NCBI Official Synonym Symbols: GIF; GLIF; MMIF
• NCBI Protein Information: macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase
• UniProt Protein Name: Macrophage migration inhibitory factor
• Protein Family: MIF-like protein
• UniProt Gene Name: MIF
• UniProt Synonym Gene Names: GLIF; MMIF; MIF; GIF
• UniProt Entry Name: MIF_HUMAN

NCBI Description

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - tyrosine; Apoptosis; Cytokine; EC 5.3.3.12; Amino Acid Metabolism - phenylalanine; Cell development/differentiation; Isomerase; EC 5.3.2.1

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle

Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; receptor binding; chemoattractant activity

Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; positive regulation of lipopolysaccharide-mediated signaling pathway; carboxylic acid metabolic process; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of MAP kinase activity; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell proliferation; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of myeloid cell apoptosis; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cytokine secretion

Disease: Rheumatoid Arthritis, Systemic Juvenile