Immunofluorescence (IF)
(Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody (MBS488657) AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 (MBS488657) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488657, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Mouse anti-Human IL-9R Monoclonal Antibody | anti-IL-9R antibody
Anti-IL-9R [AH9R2]
Immunofluorescence (IF)
(Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody (MBS488657) AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 (MBS488657) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488657, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody (MBS488657) AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 (MBS488657) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488657, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9R antibody AH9R2 (MBS488657). Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 (MBS488657, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9R antibody AH9R2 (MBS488657). Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 (MBS488657, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
This antibody was raised by immunising DBA/2 mice with P815 mastocytoma cells transfected with human IL-9R cDNA.
Note on publication: Describes the original generation of this antibody, and its use in immunoprecipitation analysis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine receptor that specifically mediates the biological effects of interleukin 9 (IL9). The functional IL9 receptor complex requires this protein as well as the interleukin 2 receptor, gamma (IL2RG), a common gamma subunit shared by the receptors of many different cytokines. The ligand binding of this receptor leads to the activation of various JAK kinases and STAT proteins, which connect to different biologic responses. This gene is located at the pseudoautosomal regions of X and Y chromosomes. Genetic studies suggested an association of this gene with the development of asthma. Multiple pseudogenes on chromosome 9, 10, 16, and 18 have been described. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
IL9R: This is a receptor for interleukin-9. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: Xq28 and Yq12
Cellular Component: extracellular space; integral to plasma membrane
Molecular Function: interleukin-9 binding; interleukin-9 receptor activity
Biological Process: cell proliferation; signal transduction; positive regulation of cell growth
Research Articles on IL-9R
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Product Notes
The IL-9R il9r (Catalog #AAA488656) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-9R [AH9R2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-9R can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Blocking (BL). This antibody has been used to immunoprecipitate human IL-9R expressed by murine BW5147 cells transfected with hIL-9R-encoding cDNA (Demoulin et al, 1996). This antibody binds to and blocks the human IL-9Ra, and displays intermediate inhibitory activity; in a TS1H9RA3 proliferation assay, half-maximal inhibition of 100 pg/ml human IL-9 requires 10 mg/ml of this antibody (de Smedt et al, 2000). Equally, treatment with this antibody partially reduces the cellularity of human thymocytes in chimeric human-mouse FTOC, with no effect on murine thymocytes (de Smedt et al, 2000). Researchers should empirically determine the suitability of the IL-9R il9r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-9R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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