Western Blot (WB)
(Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha P9108-48B (lower).)
Rabbit Protein Synthesis Initiation Factor 2 alpha Polyclonal Antibody | anti-ARALYDRAFT_908460 antibody
Protein Synthesis Initiation Factor 2 alpha, phosphorylated (Ser51) (eIF 2a, Eukaryotic Translation Initiation Factor)
Reactivity
Drosophila, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Protein Synthesis Initiation Factor 2 alpha; Polyclonal Antibody; phosphorylated (Ser51) (eIF 2a; Eukaryotic Translation Initiation Factor); Anti -Protein Synthesis Initiation Factor 2 alpha; anti-ARALYDRAFT_908460 antibody
Host
Rabbit
Reactivity
Drosophila, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous eIF2alpha only when phosphorylated at Ser51. Does not recognize elF2alpha phosphorylated at other sites.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-ARALYDRAFT_908460 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser51 of human eIF2alpha.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha P9108-48B (lower).)
Western Blot (WB)
(Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha P9108-48B (lower).)
Testing Data
Testing Data
Related Product Information for anti-ARALYDRAFT_908460 antibody
Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex. For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (5,6). This phosphorylation stabilizes the eIF2- GDP-eIF2B complex inhibiting the turnover of eIF2B. Induction of PKR by IFN-gamma and TNF-alpha, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2alpha at Ser51 (7,8).
Product Categories/Family for anti-ARALYDRAFT_908460 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ARALYDRAFT_908460 (Catalog #AAA645822) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protein Synthesis Initiation Factor 2 alpha, phosphorylated (Ser51) (eIF 2a, Eukaryotic Translation Initiation Factor) reacts with Drosophila, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Synthesis Initiation Factor 2 alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the ARALYDRAFT_908460 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Synthesis Initiation Factor 2 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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