Rabbit anti-Human NDST1 Polyclonal Antibody | anti-NDST1 antibody

NDST1, CT (NDST1, HSST, HSST1, Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, N-heparan sulfate sulfotransferase 1, [Heparan sulfate]-glucosamine N-sulfotransferase 1, Heparan sulfate N-de

Cat. Num. AAA648643

• Gene Names: NDST1; HSST; NST1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NDST1; Polyclonal Antibody; CT (NDST1; HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; N-heparan sulfate sulfotransferase 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; Heparan sulfate N-de; Anti -NDST1; anti-NDST1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NDST1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: NDST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 819-849 amino acids from the C-terminal region of human NDST1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS648643. This demonstrates that MBS648643 detected the NDST1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS648643 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-NDST1 antibody

NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.

Product Categories/Family for anti-NDST1 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 15277597
• NCBI GeneID: 3340
• UniProt Accession #: P52848; Q96E57
• Molecular Weight: 100,868 Da
• NCBI Official Full Name: NDST1 protein
• NCBI Official Synonym Full Names: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
• NCBI Official Symbol: NDST1
• NCBI Official Synonym Symbols: HSST; NST1
• NCBI Protein Information: bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; NDST-1; HSNST 1; N-HSST 1; N-heparan sulfate sulfotransferase 1; glucosaminyl N-deacetylase/N-sulfotransferase 1; heparan sulfate-N-deacetylase/N-sulfotransferase; [Heparan sulfate]-glucosamine N-sulfotransferase 1
• UniProt Protein Name: Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
• UniProt Gene Name: NDST1
• UniProt Synonym Gene Names: HSST; HSST1; NDST-1; N-HSST 1; HSNST 1
• UniProt Entry Name: NDST1_HUMAN

NCBI Description

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Function: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Ref.6 Ref.7

Catalytic activity: 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine.

Pathway: Glycan metabolism; heparan sulfate biosynthesis.Glycan metabolism; heparin biosynthesis.

Subunit structure: Monomer.

Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein Ref.2.

Tissue specificity: Widely expressed. Expression is most abundant in heart, liver and pancreas.

Miscellaneous: The presence of 4 different heparan sulfate N-deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/sulfation reactions, whereas other later on fill in or extend already modified heparan sulfate sequences.

Sequence similarities: Belongs to the sulfotransferase 1 family. NDST subfamily.

Biophysicochemical propertiesKinetic parameters:KM=13.3 µM for K5 polysaccharide Ref.7KM=0.35 µM for N-acetylated HS-II