Testing Data
Mouse anti-Human DNA Polymerase, beta Monoclonal Antibody
DNA Polymerase, beta
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
DNA Polymerase; beta; Monoclonal Antibody; Anti -DNA Polymerase; anti-DNA Polymerase; beta antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3F146
Specificity
Recognizes DNA Polymerase, beta. Species Crossreactivity: human.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See D3935-12X
Sequence
Not determined
Applicable Applications for anti-DNA Polymerase, beta antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Dilution: Western Blotting (Ab 2-4ug/ml for 2 hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4 ug/ml for 30min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4 ug/ml for 30min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Immunogen
DNA polymerase beta protein.
Cellular Localization
Nuclear
Positive Control
HeLa cells. Testis
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human testis stained with DNA-B-poly Ab-2 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human testis stained with DNA-B-poly Ab-2 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.)
Related Product Information for anti-DNA Polymerase, beta antibody
DNA polymerase
Product Categories/Family for anti-DNA Polymerase, beta antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DNA Polymerase, beta (Catalog #AAA631586) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNA Polymerase, beta reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Polymerase, beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Dilution: Western Blotting (Ab 2-4ug/ml for 2 hrs at RT) Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4 ug/ml for 30min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Researchers should empirically determine the suitability of the DNA Polymerase, beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Not determined. It is sometimes possible for the material contained within the vial of "DNA Polymerase, beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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