Mouse anti-Human Pds1 Monoclonal Antibody | anti-PDS1 antibody

Pds1

Cat. Num. AAA601156

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Pds1; Monoclonal Antibody; Anti -Pds1; anti-PDS1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3F313
• Specificity: Recognizes Pds1. Species Crossreactivity: human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See P3126X
• Sequence: Not determined

• Applicable Applications for anti-PDS1 antibody: Immunohistochemistry (IHC)
• Application Notes: Dilution: Western Blotting (Not verified), Immunohistochemistry (formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
• Immunogen: Human recombinant full length Pds1 protein
• Cellular Localization: Nuclear
• Positive Control: HeLa cells. Tonsil
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human tonsil stained with Pds1 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lympocytes in germinal center.)

Testing Data

Related Product Information for anti-PDS1 antibody

Pds1 is an anaphase inhibitor and plays important role in DNA damage and spindle check point pathways. Pds1 inhibits sister chromatid separation by binding and inhibiting Esp1, cystein protease that causes cleavage of the cohesin Scc1 that binds the sister chromatids together. Pds1 is responsible for targeting of Esp1 to nucleus and its binding to the spindle. Degradation of Pds1 occurs shortly before anaphase, which liberates Esp1 and is a prerequisite for anaphase entry. Pds1 is targeted for degradation by ubiquitination mediated by cyclosome/anaphase-promoting complex (APC) functioning as a ubiquitin ligase. In response to DNA damage Chk1 phosphorylates Pds1 to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase.

Product Categories/Family for anti-PDS1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 398365667
• NCBI GeneID: 851691
• NCBI Accession #: NP_010398.3
• NCBI GenBank Nucleotide #: NM_001180421.3
• UniProt Accession #: P40316; D6VS98
• Molecular Weight: 42kD
• NCBI Official Full Name: Pds1p
• NCBI Official Symbol: PDS1
• NCBI Protein Information: Pds1p
• UniProt Protein Name: Securin
• Protein Family: Securin
• UniProt Gene Name: PDS1
• UniProt Entry Name: SECU_YEAST

Uniprot Description

Function: Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1. Ref.6

Subunit structure: Interacts with the caspase-like ESP1, and prevents its protease activity probably by covering its active site. Interacts with CDC20. Ref.6 Ref.7

Subcellular location: Cytoplasm. Nucleus.

Domain: The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway

By similarity.

Post-translational modification: Phosphorylated by CDC28. The phosphorylation may be important for ESP1 localization to the nucleus. Ref.8Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. Ref.5

Sequence similarities: Belongs to the securin family.