SDS-Page
Ribonuclease A4 (RNASE4) Recombinant Protein | RNASE4 recombinant protein
Recombinant Ribonuclease A4 (RNASE4)
Gene Names
RNASE4; RAB1; RNS4
Reactivity
Human
Applications
SDS-Page, Western Blot
Purity
>92%
Synonyms
Ribonuclease A4 (RNASE4); Recombinant Ribonuclease A4 (RNASE4); RNASE4 recombinant protein
Host
E. coli
Reactivity
Human
Purity/Purification
>92%
Form/Format
10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose 0.01% sarcosyl and Proclin300
Concentration
Original Concentration: 200µg/mL (varies by lot)
Applicable Applications for RNASE4 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Asp30~Gly147
Tags
N-terminal His-Tag
Tissue Specificity
Pancreas
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
9.0
Predicted Molecular Mass
14.9kDa
Accurate Molecular Mass
18kDa as determined by SDS-PAGE reducing conditions
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ribonuclease 4
NCBI Official Synonym Full Names
ribonuclease, RNase A family, 4
NCBI Official Symbol
RNASE4
NCBI Official Synonym Symbols
RAB1; RNS4
NCBI Protein Information
ribonuclease 4; RNase 4; ribonuclease A B1
UniProt Protein Name
Ribonuclease 4
Protein Family
UniProt Gene Name
RNASE4
UniProt Synonym Gene Names
RNS4; RNase 4
UniProt Entry Name
RNAS4_HUMAN
NCBI Description
The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]
Uniprot Description
RNASE4: This RNase has marked specificity towards the 3' side of uridine nucleotides. Belongs to the pancreatic ribonuclease family.
Protein type: EC 3.1.27.-; Secreted, signal peptide; Ribonuclease; Secreted
Chromosomal Location of Human Ortholog: 14q11
Cellular Component: extracellular region
Molecular Function: nucleic acid binding; pancreatic ribonuclease activity
Biological Process: cellular response to starvation; mRNA cleavage
Research Articles on RNASE4
Similar Products
Product Notes
The RNASE4 rnase4 (Catalog #AAA2010588) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Ribonuclease A4 (RNASE4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ribonuclease A4 (RNASE4) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, WB (May be suitable for use in other assays to be determined by the end user.) . Researchers should empirically determine the suitability of the RNASE4 rnase4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease A4 (RNASE4), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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