Testing Data
Mouse anti-Human, Mouse Kip2, p57 Monoclonal Antibody
Kip2, p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Kip2; p57; Monoclonal Antibody; p57 (cdk Inhibitor; Cyclin Dependent Kinase Inhibitor); Anti -Kip2; anti-Kip2; p57 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3F245
Specificity
Recognizes Kip2, p57. Species Crossreactivity: human and mouse.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K1851-02X
Sequence
Not determined
Applicable Applications for anti-Kip2, p57 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Dilution: Immunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate)
Western Blotting (Not suitable)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4 ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Western Blotting (Not suitable)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4 ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Immunogen
Recombinant human p57Kip2 protein
Cellular Localization
Nuclear
Positive Control
LS174T cells. Colon carcinomas or placenta.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human colon carcinoma stained with p57 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human colon carcinoma stained with p57 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.)
Related Product Information for anti-Kip2, p57 antibody
p57Kip2 (or CDKN1C) is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57Kip2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith-Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF-II) and H19, both of which seem to be implicated in adrenal neoplasms.
Product Categories/Family for anti-Kip2, p57 antibody
Similar Products
Product Notes
The Kip2, p57 (Catalog #AAA602790) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Kip2, p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Kip2, p57 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC). Dilution: Immunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate) Western Blotting (Not suitable) Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4 ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Researchers should empirically determine the suitability of the Kip2, p57 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Not determined. It is sometimes possible for the material contained within the vial of "Kip2, p57, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
