14-3-3 eta recombinant protein

14-3-3 eta, 1-246aa, Human, His tag, E Coli

Cat. Num. AAA203144

• Gene Names: YWHAH; YWHA1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: 14-3-3 eta; 1-246aa; Human; His tag; E Coli; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta polypeptide; YWHA1; YWHAH; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta polypeptide; eta polypeptide 14 3 3 protein eta; HGNC:12853; Protein AS1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1.; 14-3-3 eta recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in 20mM Tris pH 8.0, 10% Glycerol.
• Concentration: 1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN
• Sequence Length: 246

• Applicable Applications for 14-3-3 eta recombinant protein: SDS-PAGE
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks).; For long term storage, aliquot and store at -20°C or -70°C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for 14-3-3 eta recombinant protein

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography techniques.

Product Categories/Family for 14-3-3 eta recombinant protein

Signal Transduction

References

Sato S., et al. (2006) EMBO J 25 (1):211-21; Chen J., et al. (2007), J Neurosci Res. 85 (8):1724-33;

NCBI and Uniprot Product Information

• NCBI GI #: 4507951
• NCBI GeneID: 7533
• NCBI Accession #: NP_003396
• NCBI GenBank Nucleotide #: NM_003405.3
• UniProt Accession #: Q04917
• Molecular Weight: 30.3 kDa (266 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: 14-3-3 protein eta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta
• NCBI Official Symbol: YWHAH
• NCBI Official Synonym Symbols: YWHA1
• NCBI Protein Information: 14-3-3 protein eta
• UniProt Protein Name: 14-3-3 protein eta
• UniProt Gene Name: YWHAH
• UniProt Synonym Gene Names: YWHA1

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.