Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD40 antibody 3/23 (MBS488634) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3/23 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Mouse CD40 Monoclonal Antibody | anti-CD40 antibody
Anti-CD40 [3/23]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD40 antibody 3/23 (MBS488634) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3/23 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD40 antibody 3/23 (MBS488634) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3/23 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
This antibody was raised by immunising Lou rats with murine CD40-hgamma1-Fc according to the protocol by Hasbold et al. (Eur J Immunol. 1994)
NCBI and Uniprot Product Information
Uniprot Description
CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.
Protein type: Membrane protein, integral; Receptor, cytokine
Cellular Component: extracellular space; cell surface; membrane; intracellular membrane-bound organelle; cell; cytoplasm; integral to membrane; plasma membrane; extracellular region; external side of plasma membrane
Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; antigen binding
Biological Process: positive regulation of isotype switching to IgG isotypes; regulation of immune response; B cell activation; immune system process; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; protein kinase B signaling cascade; positive regulation of B cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion
Research Articles on CD40
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Product Notes
The CD40 cd40 (Catalog #AAA488634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD40 [3/23] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD40 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP). This antibody has been used in various FACS analyses, for instance, to determine the immunoregulatory properties of primary colonic epithelial cells (Telega et al., 2000), to validate if eicosapentaenoic acid induces prolonged survival of cardiac allografts and generates regulatory T cells (Iwami et al., 2009), to characterise Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors (Zhang et al., 2006), to delineate the mechanisms for the activation of dendritic cells and induction of T cell responses by HPV 16 L1/E7 chimeric virus-like particles (Freyschmidt et al., 2004), and to investigate the role of epigenetic modification and immunomodulation in a murine prostate cancer model (Sulek et al., 2017). In a study by White et al. (J Immunol. 2011), the parental anti-mouse CD40 3/23 (rat IgG2a) mAb was first reformatted to mouse IgG1 (m1) and IgG2a (m2a) versions. The new variants were then utilised in immunohistochemistry, SPR, and in vitro phagocytosis assays and assessments for B cell proliferation, activation, and survival to confirm that interaction with FcgammaRIIB is critical for the agonistic activity of anti-CD40 amAb (White et al. 2011). Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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