Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse MAPT/Tau Monoclonal Antibody | anti-MAPT/Tau antibody
Anti-MAPT/Tau Antibody (clone MCA-2E9)
Reactivity
HumanPredicted: Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
MAPT/Tau; Monoclonal Antibody; Anti-MAPT/Tau Antibody (clone MCA-2E9); Rabbit Polyclonal to Human MAPT/Tau; MAPT; FTDP-17; MSTD; Neurofibrillary tangle protein; PHF-tau; MTBT2; PPND; DDPAC; MAPTL; MTBT1; Paired helical filament-tau; TAU; anti-MAPT/Tau antibody
Host
Mouse
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MCA-2E9
Specificity
Clone 2E9 is known to react with tau from human, mouse and rat. Since tau is highly conserved, it is likely that the antibody is effective on other species also.
Purity/Purification
Affinity Purified
Form/Format
PBS, 10 mM sodium azide.
Applicable Applications for anti-MAPT/Tau antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ICC: 1:1000
IF: 1:1000
IHC-P: 10 ug/ml
WB: 1:10000
IF: 1:1000
IHC-P: 10 ug/ml
WB: 1:10000
Immunogen
Our antibody was made against a recombinant construct expressed in and purified from E. coli and which corresponded to the shortest version of the various tau proteins, so the antibody is expected to bind to all tau isoforms.
Conjugation
Unconjugated
Preparation and Storage
Store at 4 degree C or -20 degree C. Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunocytochemistry (ICC)
(Standard immunostaining protocol (green), chicken antibody to alpha-internexin (red) and for DNA (blue). MAPT/TAU antibody stains the neuronal perikarya and process strongly, and does not stain non-neuronal cells in these cultures. The alpha-internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.)
Immunocytochemistry (ICC)
(Standard immunostaining protocol (green), chicken antibody to alpha-internexin (red) and for DNA (blue). MAPT/TAU antibody stains the neuronal perikarya and process strongly, and does not stain non-neuronal cells in these cultures. The alpha-internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.)
Western Blot (WB)
(Blots of crude rat brain extract stained with MAPT/TAU antibody, revealing, as expected, multiple bands in the range 48-67 kDa.)
Western Blot (WB)
(Blots of crude rat brain extract stained with MAPT/TAU antibody, revealing, as expected, multiple bands in the range 48-67 kDa.)
Related Product Information for anti-MAPT/Tau antibody
Tau promotes microtubule assembly and stability, and might be involvedin the establishment and maintenance of neuronal polarity. the C-terminus binds axonal microtubules while the N-terminus binds neuralplasma membrane components, suggesting that tau functions as a linkerprotein between both. Axonal polarity is predetermined by taulocalization (in the neuronal cell) in the domain of the cell body defined by the centrosome.
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Product Notes
The MAPT/Tau (Catalog #AAA2401538) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MAPT/Tau Antibody (clone MCA-2E9) reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAPT/Tau can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:1000 IF: 1:1000 IHC-P: 10 ug/ml WB: 1:10000. Researchers should empirically determine the suitability of the MAPT/Tau for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPT/Tau, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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