SDS-Page
(Up-regulation and Antibody-Peptide Competition. Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for 1 hour at RT, incubated with MBS626735 antibody for 2 hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to MBS626735 blocks the signal, verifying the specificity of the antibody.)
Rabbit anti-Human Ribosomal Protein S6 Polyclonal Antibody | anti-Despr_1827 antibody
Ribosomal Protein S6, phosphorylated (Ser244, Ser247)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Ribosomal Protein S6; Polyclonal Antibody; phosphorylated (Ser244; Ser247); Anti -Ribosomal Protein S6; anti-Despr_1827 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RPS6. Species Sequence Homology
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS pH 7.3,1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-Despr_1827 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000 dilution.
Dilution: Western Blot: 1:1000 dilution.
Immunogen
Synthetic phosphopeptide derived from the region of human RPS6 that contains serines 244 and 247. The sequence is conserved in mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Up-regulation and Antibody-Peptide Competition. Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for 1 hour at RT, incubated with MBS626735 antibody for 2 hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to MBS626735 blocks the signal, verifying the specificity of the antibody.)
SDS-Page
(Up-regulation and Antibody-Peptide Competition. Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for 1 hour at RT, incubated with MBS626735 antibody for 2 hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to MBS626735 blocks the signal, verifying the specificity of the antibody.)
Related Product Information for anti-Despr_1827 antibody
40S ribosomal protein S6 (also known as RPS6) is a ~31kD substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. Phosphorylation of RPS6 is rapamycin and wortmannin-sensitive as its activation is mediated by mTOR and PI3K pathways. Ribosomal protein S6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236 235 240 244 247, due to the positions of these amino acid residues on the -helix. Hyperphosphorylation of ribosomal protein S6 stimulates protein synthesis that mediates
Product Categories/Family for anti-Despr_1827 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
ribosomal protein S6
NCBI Official Symbol
Despr_1827
NCBI Protein Information
ribosomal protein S6
Protein Family
Similar Products
Product Notes
The Despr_1827 (Catalog #AAA626735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ribosomal Protein S6, phosphorylated (Ser244, Ser247) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ribosomal Protein S6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000 dilution. Researchers should empirically determine the suitability of the Despr_1827 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribosomal Protein S6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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