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Western Blot (WB) (Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS628373. COMP (arrow) was detected usingMBS628373.)

Rabbit anti-Human COMP Polyclonal Antibody | anti-COMP antibody

COMP, ID (Cartilage Oligomeric Matrix Protein, Thrombospondin-5, TSP5) (MaxLight 550)

Gene Names
COMP; MED; EDM1; EPD1; PSACH; THBS5
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
COMP; Polyclonal Antibody; ID (Cartilage Oligomeric Matrix Protein; Thrombospondin-5; TSP5) (MaxLight 550); anti-COMP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human COMP.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-COMP antibody
Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 314-343 in the central region of human COMP.
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS628373. COMP (arrow) was detected usingMBS628373.)

Western Blot (WB) (Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS628373. COMP (arrow) was detected usingMBS628373.)

Testing Data

(All lanes: MBS628373 (1:2000) Lane 1: Human skeletal muslce lysate Lane 2: ATDC5 whole cell lysate Lane 3: HUVEC whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MG-63 whole cell lysate Lane 6: U-2OS whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 83, 77kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data (All lanes: MBS628373 (1:2000) Lane 1: Human skeletal muslce lysate Lane 2: ATDC5 whole cell lysate Lane 3: HUVEC whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MG-63 whole cell lysate Lane 6: U-2OS whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 83, 77kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human prostate carcinoma usingMBS628373, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human prostate carcinoma usingMBS628373, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis in Jurkat cells using MBS628373 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis in Jurkat cells using MBS628373 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-COMP antibody
References
1. Kim, H.J., et.al., Eur. J. Appl. Physiol. 105(5), 765-770 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77,214 Da
NCBI Official Full Name
cartilage oligomeric matrix protein
NCBI Official Synonym Full Names
cartilage oligomeric matrix protein
NCBI Official Symbol
COMP
NCBI Official Synonym Symbols
MED; EDM1; EPD1; PSACH; THBS5
NCBI Protein Information
cartilage oligomeric matrix protein; TSP5; cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia 1, multiple); pseudoachondroplasia (epi
UniProt Protein Name
Cartilage oligomeric matrix protein
Protein Family
UniProt Gene Name
COMP
UniProt Synonym Gene Names
COMP; TSP5
UniProt Entry Name
COMP_HUMAN

NCBI Description

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; collagen binding; heparan sulfate proteoglycan binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: limb development; extracellular matrix organization and biogenesis; organ morphogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis

Disease: Epiphyseal Dysplasia, Multiple, 1; Pseudoachondroplasia

Research Articles on COMP

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Product Notes

The COMP comp (Catalog #AAA6469948) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COMP, ID (Cartilage Oligomeric Matrix Protein, Thrombospondin-5, TSP5) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COMP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the COMP comp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COMP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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