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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:FITC (MBS210754))

Mouse CD20 Monoclonal Antibody | anti-CD20 antibody

MOUSE ANTI HUMAN CD20:RPE

Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Applications
Flow Cytometry, Functional Assay
Synonyms
CD20; Monoclonal Antibody; MOUSE ANTI HUMAN CD20:RPE; anti-CD20 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2H7
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Sequence Length
297
Applicable Applications for anti-CD20 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Flow Cytometry: Maximum Dilution: Neat
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:FITC (MBS210754))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:FITC (MBS210754))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 647 (MBS212274A647))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 647 (MBS212274A647))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20 (MBS212274))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20 (MBS212274))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Biotin (MBS210355))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Biotin (MBS210355))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 488 (MBS212274A488))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 488 (MBS212274A488))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD20: Pacific Blue (MBS212274PB))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20: Pacific Blue (MBS212274PB))
Related Product Information for anti-CD20 antibody
Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells. The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
931
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,581 Da
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane-spanning 4-domains, subfamily A, member 1
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
NCBI Protein Information
B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16
UniProt Protein Name
B-lymphocyte antigen CD20
Protein Family
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: epidermal growth factor receptor binding

Biological Process: B cell proliferation; humoral immune response

Disease: Immunodeficiency, Common Variable, 5

Research Articles on CD20

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Product Notes

The CD20 ms4a1 (Catalog #AAA213656) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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