Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo
)
Mouse CytokeRatin 10+13 / KeRatin K10+K13 Monoclonal Antibody
Mouse anti CytokeRatin 10+13 / KeRatin K10+K13
Reactivity
Feline, Human, Zebrafish
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
CytokeRatin 10+13 / KeRatin K10+K13; Monoclonal Antibody; Mouse anti CytokeRatin 10+13 / KeRatin K10+K13; anti-CytokeRatin 10+13 / KeRatin K10+K13 antibody
Host
Mouse
Reactivity
Feline, Human, Zebrafish
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
DE-K13
Specificity
DE-K13 reacts with cytokeRatin 10 and 13 in fixed cells and on frozen tissues, but exclusively with cytokeRatin 13 in formalin-fixed, paraffin-embedded tissues. CytokeRatin 10 is present in keRatinizing stRatified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while cytokeRatin 13 is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stRatified epithelia, as well as carcinomas derived from these tissues.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-CytokeRatin 10+13 / KeRatin K10+K13 antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
DE-K13 is useful for immunoblotting, immunocytochemistry, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues (see also above). For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1N HCl for 30 min at room tempeRature is required. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
DE-K13 is a Mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a cytoskeletal prepaRation extracted from Human ectocervical epithelium.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo )
Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo
)
Related Product Information for anti-CytokeRatin 10+13 / KeRatin K10+K13 antibody
CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Product Categories/Family for anti-CytokeRatin 10+13 / KeRatin K10+K13 antibody
References
Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline cytokeRatins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., van Doornewaard, G., and Misdorp, W. (1993). CytokeRatins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095-102. van Bommel, P. F., Kenemans, P., Helmerhorst, T. J., Gallee, M. P., and Ivanyi, D. (1994). Expression of cytokeRatin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix, Cancer 74, 2314-20.
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Product Notes
The CytokeRatin 10+13 / KeRatin K10+K13 (Catalog #AAA570071) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CytokeRatin 10+13 / KeRatin K10+K13 reacts with Feline, Human, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's CytokeRatin 10+13 / KeRatin K10+K13 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). DE-K13 is useful for immunoblotting, immunocytochemistry, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues (see also above). For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1N HCl for 30 min at room tempeRature is required. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the CytokeRatin 10+13 / KeRatin K10+K13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CytokeRatin 10+13 / KeRatin K10+K13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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