Mouse Aggrecan Monoclonal Antibody | anti-ACAN antibody

Mouse Anti Human Aggrecan

Cat. Num. AAA225629

• Gene Names: ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
• Reactivity: Human, Bovine; Does not react with: Chicken, Fish, Rat
• Applications: Immunohistochemistry, ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Synonyms: Aggrecan; Monoclonal Antibody; Mouse Anti Human Aggrecan; Aggrecan antibody (7E1); anti-ACAN antibody

• Host: Mouse
• Reactivity: Human, Bovine; Does not react with: Chicken, Fish, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7E1
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Form/Format: Liquid; Phosphate Buffered Saline
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2415

• Applicable Applications for anti-ACAN antibody: Immunohistochemistry (IHC) Frozen, ELISA (EIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Purified human articular cartilage aggrecan
• Carrier Free: Yes
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Preservative Stabilizers: 0.09% Sodium Azide
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.; Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Published customer image: Mouse anti Human aggrecan antibody, clone 7E1 used for the detection of aggrecan expressing cells in murine micromass cultures by immunocytochemistry.Image caption: Regulation of Extracellular matrix (ECM) composition by Bone morphogenetic protein (BMP) family growth factors during chondrogenesis. Mouse micromass cultures without treatment (A-E), Gdf-5 treatment (F-J), and Bmp-7 treatment (K-O); Alcian blue stain (A-K) showed cartilage nodules, Immunohistochemical stain for type 1 collagen (A, F, K), type II collagen (C, H, M), aggrecan (D, I, N), and fibronectin (E, J, O). Statistical analysis for histochemical and immunohistochemical data, (P) = percentage of positive area for Alcian blue, type I collagen, type II collagen, aggrecan, and fibronectin stains in micromasses with different treatments; mean values are shown with Standard deviations (SD) (n = three independent experiments), One-way Analysis of variance (ANOVA). ***P )

Related Product Information for anti-ACAN antibody

Mouse anti Human aggrecan antibody, clone 7E1 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).
Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).
Mouse anti Human aggrecan antibody, clone 7E1 recognizes a ~205 kDa trypsin derived fragment of aggrecan.

Note: Originally described as being of the isotype IgM, clone 7E1 was found to be unstable and was re-cloned by the originator and the new stable sub-clone was confirmed as being an IgG1.

NCBI and Uniprot Product Information

• NCBI GI #: 129886
• NCBI GeneID: 176
• UniProt Accession #: P16112
• NCBI Official Full Name: Aggrecan core protein
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: ACAN
• NCBI Official Synonym Symbols: AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
• NCBI Protein Information: aggrecan core protein
• UniProt Protein Name: Aggrecan core protein
• Protein Family: Aggrecan core protein
• UniProt Gene Name: ACAN
• UniProt Synonym Gene Names: AGC1; CSPG1; MSK16; CSPCP
• UniProt Entry Name: PGCA_HUMAN

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region

Molecular Function: protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: keratan sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process; cell adhesion; proteolysis; skeletal development

Disease: Spondyloepiphyseal Dysplasia, Kimberley Type; Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type