Testing Data
(Brdu-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution for 1 hour at room temperature. Goat anti Mouse IgG (H/L) DyLight 649 conjugated was used as the secondary detection reagent at a 1/50 dilution. PUREBLU Hoechst 33342 (1351304) was used to stain total DNA. B. BrdU-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution overnight at 4 degree C. Goat anti Mouse IgG (H/L) FITC conjugated antibody was used at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.)
Mouse BrdU Monoclonal Antibody | anti-BrdU antibody
Mouse Anti BrdU
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Brdu-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution for 1 hour at room temperature. Goat anti Mouse IgG (H/L) DyLight 649 conjugated was used as the secondary detection reagent at a 1/50 dilution. PUREBLU Hoechst 33342 (1351304) was used to stain total DNA. B. BrdU-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution overnight at 4 degree C. Goat anti Mouse IgG (H/L) FITC conjugated antibody was used at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.)
Testing Data
(Brdu-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution for 1 hour at room temperature. Goat anti Mouse IgG (H/L) DyLight 649 conjugated was used as the secondary detection reagent at a 1/50 dilution. PUREBLU Hoechst 33342 (1351304) was used to stain total DNA. B. BrdU-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution overnight at 4 degree C. Goat anti Mouse IgG (H/L) FITC conjugated antibody was used at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.)
Testing Data
(Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to label DNA synthesis in cultured mouse astrocytes by immunofluorescence.Image caption:Astrocyte response to injury involves proliferation which is attenuated by TTX and KB-R7943. (a) GFAP positive cultured rat cortical astrocytes (green) exhibit BrdU immunolabelling (red), seen in resting cells (top row) and scratched cells (second row). There is an increase in BrdU-positive cells along the edge of a scratch, which is attenuated with TTX and KB-R7943 treatment (third and fourth rows, respectively). Scale bar 200 mum. (b) After 24 h, there was a significant 77 +/- 9% increase in proliferation amongst cells along the edge of a wound (n=7) compared to cells in unscratched cultures (n=8), as measured by BrdU staining. Compared to untreated cells, TTX decreased proliferation by 54 +/- 4%, while KB-R7943 decreased proliferation by 46 +/- 9%. *p)
Testing Data
(Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to label DNA synthesis in cultured mouse astrocytes by immunofluorescence.Image caption:Astrocyte response to injury involves proliferation which is attenuated by TTX and KB-R7943. (a) GFAP positive cultured rat cortical astrocytes (green) exhibit BrdU immunolabelling (red), seen in resting cells (top row) and scratched cells (second row). There is an increase in BrdU-positive cells along the edge of a scratch, which is attenuated with TTX and KB-R7943 treatment (third and fourth rows, respectively). Scale bar 200 mum. (b) After 24 h, there was a significant 77 +/- 9% increase in proliferation amongst cells along the edge of a wound (n=7) compared to cells in unscratched cultures (n=8), as measured by BrdU staining. Compared to untreated cells, TTX decreased proliferation by 54 +/- 4%, while KB-R7943 decreased proliferation by 46 +/- 9%. *p)
Testing Data
(Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin enhances spatial pattern separation and adult hippocampal neurogenesis in adult rats.(D) Representative images of DCX+ immature neurones and new adult-born DG neurones (white arrows) co-expressing NeuN+ and BrdU+ (yellow). Scale bar = 200mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)
Testing Data
(Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin enhances spatial pattern separation and adult hippocampal neurogenesis in adult rats.(D) Representative images of DCX+ immature neurones and new adult-born DG neurones (white arrows) co-expressing NeuN+ and BrdU+ (yellow). Scale bar = 200mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)
Testing Data
(Published Customer image:Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin does not significantly inhibit the rate of stem cell self-renewal in the SGZ of the DG of adult rats. Representative images identifying triple positive (BrdU+/Sox2+/S100B+) new adult-born astrocytes (arrows) and double-positive (BrdU+/Sox2+/S100B-) new adult-born stem cells (arrowheads). Statistical analysis was performed using one-way ANOVA with Bonferroni's post hoc test, n = 12 rats per group. Scale bar = 20mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)
Testing Data
(Published Customer image:Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin does not significantly inhibit the rate of stem cell self-renewal in the SGZ of the DG of adult rats. Representative images identifying triple positive (BrdU+/Sox2+/S100B+) new adult-born astrocytes (arrows) and double-positive (BrdU+/Sox2+/S100B-) new adult-born stem cells (arrowheads). Statistical analysis was performed using one-way ANOVA with Bonferroni's post hoc test, n = 12 rats per group. Scale bar = 20mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)
Testing Data
(Published Customer Image:Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to stain dividing crypt cells in rat small intestine by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:BrdU/ Bu20a/ Small intestine/ Rat.From: Furukawa S, Nagaike M, Ozaki K.Databases for technical aspects of immunohistochemistry.J Toxicol Pathol. 2017 Jan;30(1):79-107.)
Testing Data
(Published Customer Image:Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to stain dividing crypt cells in rat small intestine by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:BrdU/ Bu20a/ Small intestine/ Rat.From: Furukawa S, Nagaike M, Ozaki K.Databases for technical aspects of immunohistochemistry.J Toxicol Pathol. 2017 Jan;30(1):79-107.)
Testing Data
(HeLa Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with Mouse anti BrdU antibody, clone Bu20a at a dilution of 1/25. As a secondary antibody, Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used at a 1/50 dilution. Cytoplasm was stained with Rabbit anti GAPDH antibody at a dilution of 1/100. As a secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.)
Testing Data
(HeLa Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with Mouse anti BrdU antibody, clone Bu20a at a dilution of 1/25. As a secondary antibody, Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used at a 1/50 dilution. Cytoplasm was stained with Rabbit anti GAPDH antibody at a dilution of 1/100. As a secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.)
Testing Data
(Hela Cells were treated with BrdU for 3 hours and stained with Mouse anti BrdU antibody, clone Bu20a . Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used as the secondary antibody. Cytoplasm was stained with Rabbit anti GAPDH antibody . As the secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).)
Testing Data
(Hela Cells were treated with BrdU for 3 hours and stained with Mouse anti BrdU antibody, clone Bu20a . Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used as the secondary antibody. Cytoplasm was stained with Rabbit anti GAPDH antibody . As the secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).)
Testing Data
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Testing Data
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Summary: Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5?-chloro-2?-deoxyuridine (CldU), 5?-iodo-2?-deoxyuridine (IdU) and 2?-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989).
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).
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Product Notes
The BrdU (Catalog #AAA225663) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BrdU can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin. Researchers should empirically determine the suitability of the BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BrdU, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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