Flow Cytometry (FC/FACS)
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:Low Endotoxin followed by Rabbit F(ab')2 anti Mouse IgG:FITC )
Mouse anti-Human CD39 Monoclonal Antibody | anti-CD39 antibody
MOUSE ANTI HUMAN CD39: Low Endotoxin
Purified IgG - liquid
Flow Cytometry: Use 10ul of the suggested working dilutionto label 106 cells or 100ul whole blood.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Flow Cytometry (FC/FACS)
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:Low Endotoxin followed by Rabbit F(ab')2 anti Mouse IgG:FITC )
Flow Cytometry (FC/FACS)
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:Low Endotoxin followed by Rabbit F(ab')2 anti Mouse IgG:FITC )
Flow Cytometry (FC/FACS)
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39 followed by Rabbit F(ab')2 anti Mouse IgG:FITC )
Flow Cytometry (FC/FACS)
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39 followed by Rabbit F(ab')2 anti Mouse IgG:FITC )
Flow Cytometry (FC/FACS)
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:FITC )
Flow Cytometry (FC/FACS)
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:FITC )
Testing Data
(Published customer image: Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype and functional analysis of Foxp3hi and Foxp3int T cells in direct co-cultures or separated through a transwell. T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture and either re-stimulated with PMA/ionomycin for intracellular cytokine staining, or phenotyped directly. Foxp3hi:Foxp3int ratios and the percentage of IFN? or IL-4 producing cells within the CD25hiFoxp3int population were determined for 5 donors. Values for transwell-separated T cells (black bars) are expressed as fold change following normalization onto values in direct co-cultures (white bars) for each donor (Mean+/-SEM) and compared by paired Student's t-test (A). Freshly isolated CD25-Foxp3- and CD25+Foxp3+ CD4+ T cells (day 0) and CD4+CD25-Foxp3-, Foxp3int and Foxp3hi CD4+CD25hi T cells from direct or transwell separated cultures (day 6) from 3 donors were phenotyped for a wide range of markers, and expression levels for each surface marker within each population were normalized on isotype control staining, when analysed as median fluorescence intensity (MFI). Expression levels of surface markers on CD4+CD25-Foxp3- cells were comparable between conditions for each donor (data not shown). Thus, to compare the phenotype of day 0 CD25+Foxp3+ nTregs, and day 6 induced CD25hiFoxp3int and CD25hiFoxp3hi cells (black bars), expression levels are expressed as fold change (Mean+/-SEM) compared to CD4+CD25-Foxp3- cells (white bars, relative level = 1) for each donor and condition (B). Experiments were set up with a 2:1 iRBC:PBMC ratio.From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009) Plasmodium falciparum -Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFbeta.PLoS Pathog 5(8): e1000543.)
Testing Data
(Published customer image: Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype and functional analysis of Foxp3hi and Foxp3int T cells in direct co-cultures or separated through a transwell. T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture and either re-stimulated with PMA/ionomycin for intracellular cytokine staining, or phenotyped directly. Foxp3hi:Foxp3int ratios and the percentage of IFN? or IL-4 producing cells within the CD25hiFoxp3int population were determined for 5 donors. Values for transwell-separated T cells (black bars) are expressed as fold change following normalization onto values in direct co-cultures (white bars) for each donor (Mean+/-SEM) and compared by paired Student's t-test (A). Freshly isolated CD25-Foxp3- and CD25+Foxp3+ CD4+ T cells (day 0) and CD4+CD25-Foxp3-, Foxp3int and Foxp3hi CD4+CD25hi T cells from direct or transwell separated cultures (day 6) from 3 donors were phenotyped for a wide range of markers, and expression levels for each surface marker within each population were normalized on isotype control staining, when analysed as median fluorescence intensity (MFI). Expression levels of surface markers on CD4+CD25-Foxp3- cells were comparable between conditions for each donor (data not shown). Thus, to compare the phenotype of day 0 CD25+Foxp3+ nTregs, and day 6 induced CD25hiFoxp3int and CD25hiFoxp3hi cells (black bars), expression levels are expressed as fold change (Mean+/-SEM) compared to CD4+CD25-Foxp3- cells (white bars, relative level = 1) for each donor and condition (B). Experiments were set up with a 2:1 iRBC:PBMC ratio.From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009) Plasmodium falciparum -Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFbeta.PLoS Pathog 5(8): e1000543.)
Testing Data
(Published customer image: Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype of Foxp3hi and Foxp3int CD4+CD25hi T cells induced by malaria-infected RBCs. After 8 days of iRBC:PBMC co-culture, immuno magnetic selection was used to isolate CD4+CD25hi (black line) and CD25- cells (grey filled). CD25 and Foxp3 expression in the two sorted populations was analysed by flow cytometry (A). mRNA levels for Foxp3, TGFβ1 and IL-10 were normalized on 18SrRNA levels for each donor. Data from 8 donors (Mean+/-SEM) are shown, presented as relative mRNA levels normalized on mRNA levels in CD4+CD25- T cells for each donor (A). PBMC of 3 donors were phenotyped on day 0 prior to iRBC-PBMC co-culture set up, and after 6 days of iRBC:PBMC co-culture (2:1 ratio). Dot plots show the gating strategies for Foxp3 expressing cells on day 0 and day 6, respectively (B). Representative histograms depicting isotype control staining (grey filled) and specific staining for various surface markers on CD25-Foxp3- and CD25hi Foxp3-expressing CD4 T cells (black line) from each respective culture are shown (B).From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009) Plasmodium falciparum -Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFbeta.PLoS Pathog 5(8): e1000543.)
Testing Data
(Published customer image: Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype of Foxp3hi and Foxp3int CD4+CD25hi T cells induced by malaria-infected RBCs. After 8 days of iRBC:PBMC co-culture, immuno magnetic selection was used to isolate CD4+CD25hi (black line) and CD25- cells (grey filled). CD25 and Foxp3 expression in the two sorted populations was analysed by flow cytometry (A). mRNA levels for Foxp3, TGFβ1 and IL-10 were normalized on 18SrRNA levels for each donor. Data from 8 donors (Mean+/-SEM) are shown, presented as relative mRNA levels normalized on mRNA levels in CD4+CD25- T cells for each donor (A). PBMC of 3 donors were phenotyped on day 0 prior to iRBC-PBMC co-culture set up, and after 6 days of iRBC:PBMC co-culture (2:1 ratio). Dot plots show the gating strategies for Foxp3 expressing cells on day 0 and day 6, respectively (B). Representative histograms depicting isotype control staining (grey filled) and specific staining for various surface markers on CD25-Foxp3- and CD25hi Foxp3-expressing CD4 T cells (black line) from each respective culture are shown (B).From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009) Plasmodium falciparum -Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFbeta.PLoS Pathog 5(8): e1000543.)
CD39 has intrinsic ecto-ATPase activity (Wang et al. 1996), and expression can be induced on T cells and increased on B cells, as a late activation antigen (Maliszewski et al. 1994).
Mouse anti Human CD39, clone A1 has been shown to block MHC independent target cell recognition by hapten-specific CTL (Scholzen et al. 2009).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
Uniprot Description
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.
Research Articles on CD39
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Product Notes
The CD39 entpd1 (Catalog #AAA224810) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD39: Low Endotoxin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD39 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Assay, Immunofluorescence (IF). Suggested Dilution for Flow Cytometry: 1/10-1/50 Flow Cytometry: Use 10ul of the suggested working dilutionto label 106 cells or 100ul whole blood. Researchers should empirically determine the suitability of the CD39 entpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD39, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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