Testing Data
(AlexaFluor 647 conjugated Mouse anti HLA-2 antibody, clone BB7.2 used to assess HLA-2 expression on SupT1 cells by flow cytometry,Image caption:Antigen-specific elimination of target cells by sTCR-Saporin as measured by flow cytometry.(a) SupT1 cells expressing the indicated SCT were mixed at a 1:1 ratio with HLA-A2neg SupT1 cells and co-cultured for 3 days in the presence of relevant or irrelevant sTCR conjugated to Saporin, as indicated. At end of culture, cells were stained with anti-HLA-A2 AF647 to determine the frequencies of HLA-A2 positive and negative target cells among FSChi,SSChi events using flow cytometry. (b) Assay was performed as described in (a) and the percentage of SupT1 cells expressing indicated SCT that was eliminated by addition of sTCR is shown, calculated as described in materials and methods. Error bars indicate SEM of n = 3. (c) HLA-A2pos SupT1 cells mixed at a ratio of 1:1 with HLA-A2neg SupT1 cells were incubated overnight with indicated concentrations of peptide, washed, and indicated sTCR-Sap was added. SupT1 cells expressing SCT were used as positive controls (disconnected symbols). Culture and analysis to determine the percentage of eliminated HLA-A2pos targets was performed as described in (a,b). Results shown are representative of two experiments performed, and error bars represent SD of duplicates.From: Walseng E, Wälchli S, Fallang L-E, Yang W, Vefferstad A, Areffard A, et al. (2015)Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery.PLoS ONE 10(4): e0119559.)
Mouse anti-Human HLA A2 Monoclonal Antibody | anti-HLA-A antibody
Mouse Anti Human HLA A2: Amethyst Orange
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
1% Bovine Serum Albumin
This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
This product is photosensitive and should be protected from light.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(AlexaFluor 647 conjugated Mouse anti HLA-2 antibody, clone BB7.2 used to assess HLA-2 expression on SupT1 cells by flow cytometry,Image caption:Antigen-specific elimination of target cells by sTCR-Saporin as measured by flow cytometry.(a) SupT1 cells expressing the indicated SCT were mixed at a 1:1 ratio with HLA-A2neg SupT1 cells and co-cultured for 3 days in the presence of relevant or irrelevant sTCR conjugated to Saporin, as indicated. At end of culture, cells were stained with anti-HLA-A2 AF647 to determine the frequencies of HLA-A2 positive and negative target cells among FSChi,SSChi events using flow cytometry. (b) Assay was performed as described in (a) and the percentage of SupT1 cells expressing indicated SCT that was eliminated by addition of sTCR is shown, calculated as described in materials and methods. Error bars indicate SEM of n = 3. (c) HLA-A2pos SupT1 cells mixed at a ratio of 1:1 with HLA-A2neg SupT1 cells were incubated overnight with indicated concentrations of peptide, washed, and indicated sTCR-Sap was added. SupT1 cells expressing SCT were used as positive controls (disconnected symbols). Culture and analysis to determine the percentage of eliminated HLA-A2pos targets was performed as described in (a,b). Results shown are representative of two experiments performed, and error bars represent SD of duplicates.From: Walseng E, Wälchli S, Fallang L-E, Yang W, Vefferstad A, Areffard A, et al. (2015)Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery.PLoS ONE 10(4): e0119559.)
Testing Data
(AlexaFluor 647 conjugated Mouse anti HLA-2 antibody, clone BB7.2 used to assess HLA-2 expression on SupT1 cells by flow cytometry,Image caption:Antigen-specific elimination of target cells by sTCR-Saporin as measured by flow cytometry.(a) SupT1 cells expressing the indicated SCT were mixed at a 1:1 ratio with HLA-A2neg SupT1 cells and co-cultured for 3 days in the presence of relevant or irrelevant sTCR conjugated to Saporin, as indicated. At end of culture, cells were stained with anti-HLA-A2 AF647 to determine the frequencies of HLA-A2 positive and negative target cells among FSChi,SSChi events using flow cytometry. (b) Assay was performed as described in (a) and the percentage of SupT1 cells expressing indicated SCT that was eliminated by addition of sTCR is shown, calculated as described in materials and methods. Error bars indicate SEM of n = 3. (c) HLA-A2pos SupT1 cells mixed at a ratio of 1:1 with HLA-A2neg SupT1 cells were incubated overnight with indicated concentrations of peptide, washed, and indicated sTCR-Sap was added. SupT1 cells expressing SCT were used as positive controls (disconnected symbols). Culture and analysis to determine the percentage of eliminated HLA-A2pos targets was performed as described in (a,b). Results shown are representative of two experiments performed, and error bars represent SD of duplicates.From: Walseng E, Wälchli S, Fallang L-E, Yang W, Vefferstad A, Areffard A, et al. (2015)Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery.PLoS ONE 10(4): e0119559.)
Testing Data
(AlexaFluor A647 conjugated Mouse anti HLA-2 antibody, clone BB7.2 used to assess HLA-2 expression on SupT1 cells by flow cytometry,Image caption:SupT1 cells, which are HLA-A2 negative, were transduced withSCT-M1, SCT-CD20 or SCT-EBV constructs, respectively. Cells staining positively with anti-HLA-A2 were sorted, expanded and stained with anti-HLA-A2 antibodies conjugated to Alexa Fluor 647 (anti-HLA-A2-AF647), as shown. Untransduced SupT1 cells (filled grey) were used as a negative control.From: Walseng E, Wälchli S, Fallang L-E, Yang W, Vefferstad A, Areffard A, et al. (2015)Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery.PLoS ONE 10(4): e0119559.)
Testing Data
(AlexaFluor A647 conjugated Mouse anti HLA-2 antibody, clone BB7.2 used to assess HLA-2 expression on SupT1 cells by flow cytometry,Image caption:SupT1 cells, which are HLA-A2 negative, were transduced withSCT-M1, SCT-CD20 or SCT-EBV constructs, respectively. Cells staining positively with anti-HLA-A2 were sorted, expanded and stained with anti-HLA-A2 antibodies conjugated to Alexa Fluor 647 (anti-HLA-A2-AF647), as shown. Untransduced SupT1 cells (filled grey) were used as a negative control.From: Walseng E, Wälchli S, Fallang L-E, Yang W, Vefferstad A, Areffard A, et al. (2015)Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery.PLoS ONE 10(4): e0119559.)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human HLA-A2 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human HLA-A2 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.
NCBI and Uniprot Product Information
Uniprot Description
HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.
Protein type: Membrane protein, integral; Immunoglobulin superfamily
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum; Golgi apparatus; Golgi medial cisterna; Golgi membrane; integral to plasma membrane; membrane; MHC class I protein complex; phagocytic vesicle membrane; plasma membrane
Molecular Function: beta-2-microglobulin binding; peptide antigen binding; protein binding; receptor binding; T cell receptor binding; TAP binding
Biological Process: antibacterial humoral response; antigen processing and presentation; antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; defense response to Gram-positive bacterium; detection of bacterium; immune response; positive regulation of interferon-gamma production; positive regulation of T cell cytokine production; positive regulation of T cell mediated cytotoxicity; protection from natural killer cell mediated cytotoxicity; regulation of defense response to virus by virus; regulation of immune response; viral reproduction
Disease: Severe Cutaneous Adverse Reaction, Susceptibility To
Similar Products
Product Notes
The HLA-A hla-a (Catalog #AAA225862) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human HLA A2: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA A2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Researchers should empirically determine the suitability of the HLA-A hla-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA A2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
