MDGA2 recombinant protein

MDGA2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122739

• Gene Names: Mdga2; Adp; Mamdc1; 6720489L24Rik; 9330209L04Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: MDGA2; MDGA2 Protein; Mouse; Recombinant (His Tag); Mouse MDGA2/MAMDC1 Protein (His Tag); 6720489L24Rik Protein; 9330209L04Rik Protein; Adp Protein; Mamdc1 Protein; MAM domain containing glycosylphosphatidylinositol anchor 2; MDGA2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp924

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Pro 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse MDGA2 isoform 1 (P60755-1) without the propeptide (Met 1-Asp 924) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MDGA2 recombinant protein

Background: MAM domain-containing glycosylphosphatidylinositol anchor protein 2, also known as MAM domain-containing protein 1, MDGA2, and MAMDC1, is a cell membrane protein that contains six Ig-like (immunoglobulin-like) domains and one MAM domain. Analyses of the full-length coding region of MDGA1 and MDGA2 indicate that they encode proteins that comprise a novel subgroup of the Ig superfamily and have a unique structural organization consisting of six immunoglobulin (Ig)-like domains followed by a single MAM domain. Biochemical characterization demonstrates that MDGA1 and MDGA2 proteins are highly glycosylated and that MDGA1 is tethered to the cell membrane by a GPI anchor. The MDGAs are differentially expressed by subpopulations of neurons in both the central and peripheral nervous systems, including neurons of the basilar pons, inferior olive, cerebellum, cerebral cortex, olfactory bulb, spinal cord, and dorsal root and trigeminal ganglia. The similarity of MDGAs to other Ig-containing molecules and their temporal-spatial patterns of expression within restricted neuronal populations, for example migrating pontine neurons and D1 spinal interneurons, suggest a role for these novel proteins in regulating neuronal migration, as well as other aspects of neural development, including axon guidance.

References

Litwack,ED. et al., 2004, Mol Cell Neurosci. 25 (2): 263-74. Hellquist, A. et al., 2009, PLoS One. 4 (12): e8037. Sano,S. et al., 2009, Genesis. 47 (8):505-13. Bucan, M. et al., 2009, PLoS Genet. 5 (6):e1000536.

NCBI and Uniprot Product Information

• NCBI GI #: 514961954
• NCBI GeneID: 320772
• NCBI Accession #: NP_996893.3
• NCBI GenBank Nucleotide #: NM_207010.2
• UniProt Accession #: P60755
• Molecular Weight: 106,689 Da
• NCBI Official Full Name: MAM domain-containing glycosylphosphatidylinositol anchor protein 2 isoform B
• NCBI Official Synonym Full Names: MAM domain containing glycosylphosphatidylinositol anchor 2
• NCBI Official Symbol: Mdga2
• NCBI Official Synonym Symbols: Adp; Mamdc1; 6720489L24Rik; 9330209L04Rik
• NCBI Protein Information: MAM domain-containing glycosylphosphatidylinositol anchor protein 2; acrodysplasia; MAM domain containing 1
• UniProt Protein Name: MAM domain-containing glycosylphosphatidylinositol anchor protein 2
• Protein Family: MAM domain-containing glycosylphosphatidylinositol anchor protein
• UniProt Gene Name: Mdga2
• UniProt Synonym Gene Names: Mamdc1
• UniProt Entry Name: MDGA2_MOUSE