CCDC134 recombinant protein

CCDC134 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121306

• Gene Names: CCDC134; dJ821D11.3
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CCDC134; CCDC134 Protein; Human; Recombinant (His Tag); Human CCDC134 Protein (His Tag); coiled-coil domain containing 134; CCDC134 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu229

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CCDC134 (Q9H6E4)(Met 1-Leu 229) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCDC134 recombinant protein

Background: Coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.

References

Huang J, et al. (2007) CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK. Cellular and Molecular Life Sciences. 65(2): 338-49.Kim S, et al. (2011) Genome-wide association study of CSF biomarkers Abeta1-42, t-tau, and p-tau181p in the ADNI cohort. Neurology. 76(1): 69-79.

NCBI and Uniprot Product Information

• NCBI GI #: 13376216
• NCBI GeneID: 79879
• NCBI Accession #: NP_079097
• NCBI GenBank Nucleotide #: NM_024821.2
• UniProt Accession #: Q9H6E4
• Molecular Weight: 26,561 Da
• NCBI Official Full Name: coiled-coil domain-containing protein 134
• NCBI Official Synonym Full Names: coiled-coil domain containing 134
• NCBI Official Symbol: CCDC134
• NCBI Official Synonym Symbols: dJ821D11.3
• NCBI Protein Information: coiled-coil domain-containing protein 134
• UniProt Protein Name: Coiled-coil domain-containing protein 134
• Protein Family: Coiled-coil domain-containing protein
• UniProt Gene Name: CCDC134
• UniProt Entry Name: CC134_HUMAN

Uniprot Description

CCDC134: Belongs to the UPF0388 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: membrane; extracellular region