MDGA2 / MAMDC1 recombinant protein

Recombinant Mouse MDGA2 / MAMDC1 Protein (His tag)

Cat. Num. AAA2546986

• Gene Names: Mdga2; Adp; Mamdc1; 6720489L24Rik; 9330209L04Rik
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: MDGA2 / MAMDC1; Recombinant Mouse MDGA2 / MAMDC1 Protein (His tag); 6720489L24Rik; 9330209L04Rik; Mamdc1; Mdga2; MDGA2 / MAMDC1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 949

• Application Notes: The secreted recombinant mouse MDGA2 comprises 910 amino acids and has a predicted molecular mass of 103 kDa. As a result of glycosylation, the apparent molecular mass of rm MDGA2 is approximately 110-120 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Pro 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MDGA2 / MAMDC1 recombinant protein

Background: Mouse MAM domain-containing glycosylphosphatidylinositol anchor protein 2, also known as MAM domain-containing protein 1, MDGA2 and MAMDC1, is a cell membrane protein which contains six Ig-like (immunoglobulin-like) domains and one MAM domain. Analyses of the full-length coding region of MDGA1 and MDGA2 indicate that they encode proteins that comprise a novel subgroup of the Ig superfamily and have a unique structural organization consisting of six immunoglobulin (Ig)-like domains followed by a single MAM domain. Biochemical characterization demonstrates that MDGA1 and MDGA2 proteins are highly glycosylated, and that MDGA1 is tethered to the cell membrane by a GPI anchor. The MDGAs are differentially expressed by subpopulations of neurons in both the central and peripheral nervous systems, including neurons of the basilar pons, inferior olive, cerebellum, cerebral cortex, olfactory bulb, spinal cord, and dorsal root and trigeminal ganglia. The similarity of MDGAs to other Ig-containing molecules and their temporal-spatial patterns of expression within restricted neuronal populations, for example migrating pontine neurons and D1 spinal interneurons, suggest a role for these novel proteins in regulating neuronal migration, as well as other aspects of neural development, including axon guidance.

Description: A DNA sequence encoding the mouse MDGA2 isoform 1 (P60755-1) without the propeptide (Met 1-Asp 924) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 514961954
• NCBI GeneID: 320772
• NCBI Accession #: NP_996893.3
• NCBI GenBank Nucleotide #: NM_207010.2
• UniProt Accession #: P60755
• Molecular Weight: 106,689 Da
• NCBI Official Full Name: MAM domain-containing glycosylphosphatidylinositol anchor protein 2 isoform B
• NCBI Official Synonym Full Names: MAM domain containing glycosylphosphatidylinositol anchor 2
• NCBI Official Symbol: Mdga2
• NCBI Official Synonym Symbols: Adp; Mamdc1; 6720489L24Rik; 9330209L04Rik
• NCBI Protein Information: MAM domain-containing glycosylphosphatidylinositol anchor protein 2
• UniProt Protein Name: MAM domain-containing glycosylphosphatidylinositol anchor protein 2
• UniProt Gene Name: Mdga2
• UniProt Synonym Gene Names: Mamdc1

Uniprot Description

MAMDC1: May involved in cell-cell interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 12 C2|12 27.63 cM

Biological Process: pattern specification process