HepaCAM2 recombinant protein

HEPACAM2 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123395

• Gene Names: Hepacam2; RGD1305697
• Purity: >95% as determined by SDS-PAGE
• Synonyms: HEPACAM2; HEPACAM2 Protein; Rat; Recombinant (His Tag); Rat HepaCAM2 Protein (His Tag); HepaCAM2 Protein; HEPACAM family member 2; HepaCAM2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu350

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 33
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat HepaCAM2 (B5DEN8)(Met1-Leu350) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HepaCAM2 recombinant protein

Background: HEPACAM2 (HEPACAM Family Member 2) is a Protein Coding gene. This gene encodes a protein related to the immunoglobulin superfamily that plays a role in mitosis. Knockdown of this gene results in prometaphase arrest, abnormal nuclear morphology, and apoptosis. HepaCAM2, together with HEPACAM1, are cell adhesion molecules. HEPACAM1 modulates cell adhesion and migration. It also inhibits cancer cell growth. HepaCAM2 contains 2 Ig-like C2-type (immunoglobulin-like) domains. The immunoglobulin domain is a type of protein domain that consists of a 2-layer sandwich of between 7 and 9 antiparallel beta-strands arranged in two beta-sheets with a Greek key topology. Members of the immunoglobulin superfamily are found in hundreds of proteins of different functions.

References

Moh MC, et al. (2008) Expression of hepaCAM is downregulated in cancers and induces senescence-like growth arrest via a p53/p21-dependent pathway in human breast cancer cells. Carcinogenesis. 29(12):2298-305.Moh MC, et al. (2005) Structural and functional analyses of a novel ig-like cell adhesion molecule, hepaCAM, in the human breast carcinoma MCF7 cells. J Biol Chem. 280(29):27366-74.Chung Moh M, et al. (2005) Cloning and characterization of hepaCAM, a novel Ig-like cell adhesion molecule suppressed in human hepatocellular carcinoma. J Hepatol. 42(6):833-41.

NCBI and Uniprot Product Information

• NCBI GI #: 157819115
• NCBI GeneID: 296846
• NCBI Accession #: NP_001100050.1
• NCBI GenBank Nucleotide #: NM_001106580.2
• UniProt Accession #: B5DEN8
• Molecular Weight: 51,211 Da
• NCBI Official Full Name: HEPACAM family member 2
• NCBI Official Synonym Full Names: HEPACAM family member 2
• NCBI Official Symbol: Hepacam2
• NCBI Official Synonym Symbols: RGD1305697
• NCBI Protein Information: HEPACAM family member 2
• UniProt Protein Name: HEPACAM family member 2
• Protein Family: HEPACAM family
• UniProt Gene Name: Hepacam2

Uniprot Description

HEPACAM2: Required during prometaphase for centrosome maturation. Following poly-ADP-ribosylation (PARsylation) by TNKS, translocates from the Golgi apparatus to mitotic centrosomes and plays a key role in the formation of robust microtubules for prompt movement of chromosomes: anchors AKAP9/CG-NAP, a scaffold protein of the gamma-tubulin ring complex and promotes centrosome maturation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13

Cellular Component: centrosome; Golgi apparatus; midbody; spindle

Biological Process: centrosome organization and biogenesis