NEGR1 recombinant protein

NEGR1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122863

• Gene Names: Negr1; Ntra; KILON; 5330422G01Rik
• Purity: >94% as determined by SDS-PAGE
• Synonyms: NEGR1; NEGR1 Protein; Mouse; Recombinant (His Tag); Mouse NEGR1 Protein (His Tag); 5330422G01Rik Protein; KILON Protein; Ntra Protein; neuronal growth regulator 1; NEGR1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly318

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse NEGR1 (NP_001034183.1)(Met 1-Gly 318) without the pro peptide was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NEGR1 recombinant protein

Background: Neuronal Growth Regulator 1, NEGR1, also known as neurotractin, or KILON, belongs to the immunoglobulin superfamily, IgLON family. This GPI-linked cell surface glycoprotein NEGR1 is composed of three Ig-like domains and belongs to the IgLON subgroup of neural IgSF members. It is expressed in two isoforms with apparent molecular masses of 50 and 37 kD, termed L-form and S-form, respectively. NEGR1/Neurotractin participates in the regulation of neurite outgrowth in the developing brain and is expressed on the neurites of primary hippocampal neurons. Neurotractin/KILON is a trans-neural growth-promoting factor for outgrowing axons following hippocampal denervation. KILON (kindred of IgLON) and opioid-binding cell adhesion molecule, together with the limbic system-associated membrane protein and neurotrophin, belong to the IgLON subgroup of immunoglobulin superfamily. The alteration of the modulatory function of KILON/NEGR1 for the number of dendritic synapses concomitant with changes in its localization and detergent solubility during neuronal culture development. In addition to its reported role in the brain, NEGR1 is also expressed in subcutaneous adipose tissue and acts as a central 'hub' in an obesity-related transcript network.

References

Marg A, et al. (1999) Neurotractin, a novel neurite outgrowth-promoting Ig-like protein that interacts with CEPU-1 and LAMP. J Cell Biol. 145(4): 865-76.Miyata S, et al. (2003) Biochemical and ultrastructural analyses of IgLON cell adhesion molecules, Kilon and OBCAM in the rat brain. Neuroscience. 117(3): 645-58.Schfer M, et al. (2005) Neurotractin/kilon promotes neurite outgrowth and is expressed on reactive astrocytes after entorhinal cortex lesion. Mol Cell Neurosci. 29(4): 580-90.Hashimoto T, et al. (2008) IgLON cell adhesion molecule Kilon is a crucial modulator for synapse number in hippocampal neurons. Brain Res. 1224: 1-11.

NCBI and Uniprot Product Information

• NCBI GI #: 84875493
• NCBI GeneID: 320840
• NCBI Accession #: NP_001034183.1
• NCBI GenBank Nucleotide #: NM_001039094.3
• UniProt Accession #: Q80Z24; Q3UHQ8; Q80T70
• Molecular Weight: 37,900 Da
• NCBI Official Full Name: neuronal growth regulator 1 isoform a
• NCBI Official Synonym Full Names: neuronal growth regulator 1
• NCBI Official Symbol: Negr1
• NCBI Official Synonym Symbols: Ntra; KILON; 5330422G01Rik
• NCBI Protein Information: neuronal growth regulator 1; neurotractin; kindred of IgLON
• UniProt Protein Name: Neuronal growth regulator 1
• Protein Family: Neuronal growth regulator
• UniProt Gene Name: Negr1
• UniProt Synonym Gene Names: Kiaa3001; Ntra; Kilon
• UniProt Entry Name: NEGR1_MOUSE

Uniprot Description

NEGR1: May be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain. Belongs to the immunoglobulin superfamily. IgLON family.

Protein type: Membrane protein, GPI anchor

Cellular Component: neuron projection; cell surface; cell soma; membrane; dendrite; plasma membrane; intercellular junction

Biological Process: cell-cell adhesion; locomotory behavior; feeding behavior; cell adhesion