Flow Cytometry (FC/FACS)
Eukaryotes Calcium Detection Kit
EZCell Calcium Detection Kit (Cell-Based)
Reactivity
Eukaryotes
Synonyms
Calcium; EZCell Calcium Detection Kit (Cell-Based); Calcium detection kit
Reactivity
Eukaryotes
Samples
Suspension or adherent cells cultures
Detection Method
Fluorescence Microscopy/Flow Cytometer: FL1
Features & Benefits
Sensitive Cell-Based Assay for determination of intracellular calcium
Simple procedure; takes ~ 2 hours
Fast and convenient
The assay is sensitive, stable, specific and high-throughput adaptable
the kit can visualize intracellular calcium content in suspension and/or adherent cells
Simple procedure; takes ~ 2 hours
Fast and convenient
The assay is sensitive, stable, specific and high-throughput adaptable
the kit can visualize intracellular calcium content in suspension and/or adherent cells
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Related Product Information for Calcium detection kit
Calcium is essential for all living organisms. Calcium sequestration and release into the cytoplasm function as a signal for many cellular processes. As an important second messenger, calcium is involved in many physiological processes such as nerve impulse transmission, muscle contraction, etc. Intracellular calcium is mainly stored in mitochondria and endoplasmic reticulum, and its concentration in cytosol is tightly regulated. Dysfunction of calcium homeostasis causes oxidative stress, cell death and pathogenesis of Alzheimer's disease. has developed an EZCell Calcium Staining Kit that contains membrane permeable, intracellular calcium probe. Upon cell entry, the calcium probe is hydrolyzed by intracellular esterases rendering it membrane impermeable. The cleaved calcium probe binds to intracellular calcium generating fluorescence. The intensity of fluorescence is directly proportional to intracellular calcium levels. A Positive Control reagent is also provided, and serves as experimental control, which elevates intracellular Calcium levels resulting in increased calcium staining. This easy-to-use, non-radioactive kit allows studying the regulation of calcium at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells
Product Categories/Family for Calcium detection kit
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Product Notes
The Eukaryotes Calcium (Catalog #AAA849657) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849657 Detection Kit recognizes Eukaryotes Calcium. It is sometimes possible for the material contained within the vial of "Calcium, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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