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Immunocytochemistry/Immunofluorescence (ICC/IF))

Eukaryotes Intracellular Zinc Staining Kit

EZCell Intracellular Zinc Staining Kit (Cell-Based)

Reactivity
Eukaryotes
Synonyms
Intracellular Zinc; EZCell Intracellular Zinc Staining Kit (Cell-Based); Intracellular Zinc staining kit
Ordering
For Research Use Only!
Reactivity
Eukaryotes
Samples
Suspension or adherent cells cultures
Detection Method
Fluorescence Microscopy/Flow Cytometer: FL1
Features & Benefits
Sensitive Cell-Based Assay for determination of intracellular zinc
Simple procedure; takes ~ 2 hours
Fast and convenient
The assay is sensitive, stable, specific and high-throughput adaptable
Applications- the kit can visualize intracellular content in suspension and/or adherent cells
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months

Immunocytochemistry/Immunofluorescence (ICC/IF))

Immunocytochemistry/Immunofluorescence (ICC/IF))

Testing Data

Testing Data
Related Product Information for Intracellular Zinc staining kit
Zinc (Zn, Atomic Number: 30) is an essential mineral of great biological significance. It is a metal with characteristics similar to Magnesium due to its size and +2 oxidation state. It is the second most abundant transition metal in living organisms after iron. Zn2+ binding proteins account for nearly 50% of the transcription regulatory proteins in the human genome and require zinc as an essential cofactor in the regulation of gene expression. Numerous enzymes also employ Zn2+ ions in their active sites. Free Zn2+ is released from metalloprotein complexes during oxidative stress with the remainder being bound to proteins or nucleic acids. Other examples of biological roles of Zinc include signal transduction, regulation of apoptosis, neurotransmission, synaptic plasticity and prostate gland function. In humans, Zinc deficiency is associated with many chronic diseases such as renal disease, sickle cell anemia, cirrhosis, some cancers and deficiencies in metabolism. has developed the EZCell Intracellular Zinc Staining Kit (cell-based) which contains a selective and membrane permeable fluorescent probe for detection of intracellular Zinc. We also include a Positive Control that elevates Zinc staining, and cell-permeable Zinc Chelator that reduces fluorescence induced by Positive Control. This easy-to-use non-radioactive assay allows studies of the regulation of Zinc at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells

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Product Notes

The Eukaryotes Intracellular Zinc (Catalog #AAA849716) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849716 Staining Kit recognizes Eukaryotes Intracellular Zinc. It is sometimes possible for the material contained within the vial of "Intracellular Zinc, Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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