Mouse P38 Mitogen-activated Protein Kinase ELISA Kit | MAPK P38 elisa kit

Mouse P38 Mitogen-activated Protein Kinase ELISA Kit

Cat. Num. AAA764658

• Gene Names: MAPK13; SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
• Reactivity: Mouse
• Synonyms: P38 Mitogen-activated Protein Kinase; Mouse P38 Mitogen-activated Protein Kinase ELISA Kit; MAPK P38; MAPK P38 elisa kit

• Reactivity: Mouse
• Sequence Length: 365

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.25-80ng/ml
• Sensitivity: 0.75ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MAPK P38 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse MAPK P38 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse MAPK P38 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MAPK P38 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse MAPK P38 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2360960
• NCBI GeneID: 5603
• Molecular Weight: 28,779 Da
• NCBI Official Full Name: p38 mitogen-activated protein kinase
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 13
• NCBI Official Symbol: MAPK13
• NCBI Official Synonym Symbols: SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
• NCBI Protein Information: mitogen-activated protein kinase 13
• UniProt Protein Name: Mitogen-activated protein kinase 13
• UniProt Gene Name: MAPK13
• UniProt Synonym Gene Names: PRKM13; SAPK4; MAP kinase 13; MAPK 13; MAP kinase p38 delta
• UniProt Entry Name: MK13_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein (MAP) kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The encoded protein is a p38 MAP kinase and is activated by proinflammatory cytokines and cellular stress. Substrates of the encoded protein include the transcription factor ATF2 and the microtubule dynamics regulator stathmin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

P38D: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have a few hundred substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of tau and STH. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator Myb in response to stress which leads to rapid Myb degradation via a proteasome-dependent pathway. Phosphorylates and down-regulates PKD1 during regulation of insulin secretion in pancreatic beta cells. Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells. This description may include information from UniProtKB

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; MAPK/p38 subfamily; p38 subfamily

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: cytosol

Molecular Function: ATP binding; MAP kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: cell cycle; MAPKKK cascade; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of inflammatory response; positive regulation of interleukin-6 production; Ras protein signal transduction; regulation of transcription, DNA-dependent; response to osmotic stress; response to stress; transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway