Bovine Galectin-1 ELISA Kit | LGALS1 elisa kit

Bovine Galectin-1 ELISA Kit

Cat. Num. AAA2882620

• Reactivity: Bovine
• Synonyms: Galectin-1; Bovine Galectin-1 ELISA Kit; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1; LGALS1; LGALS1 elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural bovineGal-1. No significant cross-reactivity or interference was observed.
• Sequence Length: 135

• Samples: Serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofbovineGal-1concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gal-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGal-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gal-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Gal-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 28461189
• NCBI GeneID: 326598
• NCBI Accession #: NP_786976.1
• NCBI GenBank Nucleotide #: NM_175782.1
• UniProt Accession #: P11116; P11945; Q54A27
• Molecular Weight: 14,744 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Symbol: LGALS1
• NCBI Protein Information: galectin-1
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1
• UniProt Entry Name: LEG1_BOVIN

Uniprot Description

Lectin that binds beta-galactoside and a wide array of complex carbohydrates (PubMed:1900835, PubMed:8108426, PubMed:7773775). Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.