Mouse Galectin-1 ELISA Kit | LGALS1 elisa kit

Mouse Galectin-1 PicoKine ELISA Kit

Cat. Num. AAA175783

• Gene Names: Lgals1; L14; Gal-1; Galbp; L-14.5; Lect14; AA410090; galectin-1
• Reactivity: Mouse
• Synonyms: Galectin-1; Mouse Galectin-1 PicoKine ELISA Kit; lectin; galactoside-binding; soluble; 1; 14 kDa laminin-binding protein; 14 kDa lectin; Beta galactoside binding protein 14kDa; Beta-galactoside-binding lectin L-14-I; Gal-1; GAL1; Galaptin; GBP; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; LEG1_HUMAN; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1; LGALS1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Galectin-1
• Sequence Length: 135

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 5pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Galectin-1 PicoKine ELISA Kit standard curve )

Related Product Information for LGALS1 elisa kit

Principle of the assay: mouse Galectin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-1 has been precoated onto 96-well plates. Standards (E.coli,A2-E135) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-1 amount of sample captured in plate.
Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse.[4] They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 126172
• NCBI GeneID: 16852
• UniProt Accession #: P16045; P05163; P11946; P17601; Q4FZH4; Q99M27; Q9D0X0
• Molecular Weight: 14,866 Da
• NCBI Official Full Name: Galectin-1
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 1
• NCBI Official Symbol: Lgals1
• NCBI Official Synonym Symbols: L14; Gal-1; Galbp; L-14.5; Lect14; AA410090; galectin-1
• NCBI Protein Information: galectin-1; galaptin; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; beta-galactoside binding protein; lectin galactoside-binding soluble 1; beta-galactoside-binding lectin L-14-I
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: Lgals1
• UniProt Synonym Gene Names: Gbp; Gal-1
• UniProt Entry Name: LEG1_MOUSE

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; intracellular; nucleus

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; lactose binding; galactose binding; laminin binding; galactoside binding; carbohydrate binding; glycoprotein binding

Biological Process: heterophilic cell adhesion; myoblast differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; plasma cell differentiation; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; signal transduction