Rabbit VPS29 Polyclonal Antibody | anti-VPS29 antibody

VPS29 Antibody

Cat. Num. AAA7053341

• Gene Names: VPS29; DC7; DC15; PEP11
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: VPS29; Polyclonal Antibody; VPS29 Antibody; Vacuolar protein sorting-associated protein 29; hVPS29; PEP11 homolog; Vesicle protein sorting 29; DC15; DC7; MDS007; anti-VPS29 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 182

• Applicable Applications for anti-VPS29 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human Vacuolar protein sorting-associated protein 29 protein (68-145AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: MCF-7 whole cell lysate,HepG2 whole cell lysate,Hela whole cell lysate,Mouse heart tissue,Mouse brain tissue,Rat liver tissue,Rat kidney tissueAll lanes: VPS29 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 21 KDaObserved band size: 28 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7053341 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of PC3 cells using MBS7053341 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-VPS29 antibody

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (Probable). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with ANKRD27 (PubMed:24856514).

NCBI and Uniprot Product Information

• NCBI GI #: 7706441
• NCBI GeneID: 51699
• NCBI Accession #: NP_057310.1
• NCBI GenBank Nucleotide #: NM_016226.4
• UniProt Accession #: Q9UBQ0; Q502Y5; Q6FIF8; Q6IAH3; Q9H0W0; Q9NRP1; Q9NRU7
• Molecular Weight: 20,927 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 29 isoform 1
• NCBI Official Synonym Full Names: VPS29, retromer complex component
• NCBI Official Symbol: VPS29
• NCBI Official Synonym Symbols: DC7; DC15; PEP11
• NCBI Protein Information: vacuolar protein sorting-associated protein 29
• UniProt Protein Name: Vacuolar protein sorting-associated protein 29
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS29
• UniProt Synonym Gene Names: hVPS29

NCBI Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

VPS29: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). Belongs to the VPS29 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.3; Protein phosphatase, Ser/Thr (non-receptor); Vesicle

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: cytoplasm; cytosol; endosome; intracellular membrane-bound organelle; retromer complex

Molecular Function: protein binding; protein transporter activity

Biological Process: intracellular protein transport; retrograde transport, endosome to Golgi; Wnt receptor signaling pathway