Human Galectin-1 ELISA Kit | LGALS1 elisa kit

Human Galectin-1 ELISA Kit

Cat. Num. AAA765403

• Gene Names: LGALS1; GBP; GAL1
• Reactivity: Human
• Synonyms: Galectin-1; Human Galectin-1 ELISA Kit; GAL1 (Galectin 1 )/LGALS1/BHL/Galaptin/GBP/L-14/14 kDa laminin-binding protein/Gal-1/galectin 1/galectin-1/GBP/HBL/HLBP14/HPL/Lactose-binding lectin 1; LGALS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LGALS1. No significant cross-reactivity or interference between LGALS1 and analogues was observed.
• Sequence Length: 135

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI Accession #: NP_002296.1
• NCBI GenBank Nucleotide #: NM_002305.3
• Molecular Weight: 14,716 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Secreted; Extracellular matrix; Cell adhesion

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular space; intracellular; nucleus; proteinaceous extracellular matrix

Molecular Function: glycoprotein binding; lactose binding; laminin binding; protein binding; protein homodimerization activity; signal transducer activity

Biological Process: apoptosis; multicellular organismal response to stress; myoblast differentiation; plasma cell differentiation; positive regulation of erythrocyte aggregation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of virion penetration into host cell; regulation of apoptosis; response to axon injury; response to drug; signal transduction; T cell costimulation