Rabbit CD34 Polyclonal Antibody | anti-CD34 antibody

Anti-CD34 antibody

Cat. Num. AAA355115

• Reactivity: Human, mouse, rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Immunogen affinity purified
• Synonyms: CD34; Polyclonal Antibody; Anti-CD34 antibody; Polyclonal Anti- CD34; anti-CD34 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-CD34 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB (1:5000); ICC (1:250)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of human CD34.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

Related Product Information for anti-CD34 antibody

CD34 (Hematopoietic progenitor cell antigen CD34) is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue.1 It plays an essential role in early hematopoiesis.2 CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia whereas the L-selectin to which it binds is on the T cell.3,4

References

1. Nielsen JS, McNagny KM (2008). "Novel functions of the CD34 family". J of Cell Science 121 (Pt 22): 3682-3692. doi:10.1242/jcs.037507 2. He, X.-Y., Antao, V. P., Basila, D., Marx, J. C., Davis, B. R. Isolation and molecular characterization of the human CD34 gene. Blood 79: 2296-2302, 1992. 3. Berg EL, Mullowney AT, Andrew DP, Goldberg JE, Butcher EC (February 1998). "Complexity and differential expression of carbohydrate epitopes associated with L-selectin recognition of high endothelial venules". Am. J. Pathol. 152 (2): 469-77. 4. SSuzawa K, Kobayashi M, Sakai Y, Hoshino H, Watanabe M, Harada O, Ohtani H, Fukuda M, Nakayama J (July 2007). "Preferential induction of peripheral lymph node addressin on high endothelial venule-like vessels in the active phase of ulcerative colitis". Am. J. Gastroenterol. 102 (7): 1499-509.

NCBI and Uniprot Product Information

• NCBI GI #: 264769
• NCBI GeneID: 947
• UniProt Accession #: P28906; Q15970; Q15971; Q5JTA3; Q5JTA4; Q9UJB1; A8K664
• Molecular Weight: 40,716 Da
• NCBI Official Full Name: CD34
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34; CD34 antigen
• UniProt Protein Name: Hematopoietic progenitor cell antigen CD34
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: CD34
• UniProt Entry Name: CD34_HUMAN

NCBI Description

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Developmental stage: On early hematopoietic progenitor cells.

Post-translational modification: Highly glycosylated.Phosphorylated on serine residues by PKC. Ref.10

Sequence similarities: Belongs to the CD34 family.

Sequence caution: The sequence AAA03181.1 differs from that shown. Reason: Erroneous initiation.