TBX3 elisa kit

TBX3 TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502265

• Gene Names: TBX3; UMS; XHL; TBX3-ISO
• Reactivity: Human, Mouse, Rat
• Synonyms: TBX3; TBX3 TFact DNA-Binding ELISA Kit; T-box protein 3; T-box transcription factor TBX3; TBX3 elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The TFact TBX3 DNA-Binding ELISA Kit detects endogenous levels of TBX3.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 723

• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact TBX3 DNA-Binding ELISA detects active TBX3 in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

Related Product Information for TBX3 elisa kit

Principle of the Assay: The TFactTM DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample. Currently, the most common methods to detect transcription factor binding to DNA elements and motifs are electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation, western blotting, and expression of fused target and reporter genes. These methods are often time consuming, complicated, and make it difficult to achieve satisfactory results. TFact DNA-Binding ELISA Kits can significantly reduce the necessary runtime to within one day and eliminate the need for harmful radioactive labeling while maintaining high sensitivity and signal-to-noise ratio. In the past, it was strenuous and inefficient to perform high-throughput screening for hundreds of different samples or transcription factors. Today, our revolutionary TFact DNA-Binding ELISA Kits can eliminate these challenges and help expedite the journey from research to publication or product.

NCBI and Uniprot Product Information

• NCBI GI #: 47419905
• NCBI GeneID: 6926
• NCBI Accession #: NP_005987.3
• NCBI GenBank Nucleotide #: NM_005996.3
• UniProt Accession #: O15119; Q8TB20; Q9UKF8
• Molecular Weight: – Da
• NCBI Official Full Name: T-box transcription factor TBX3 isoform 1
• NCBI Official Synonym Full Names: T-box 3
• NCBI Official Symbol: TBX3
• NCBI Official Synonym Symbols: UMS; XHL; TBX3-ISO
• NCBI Protein Information: T-box transcription factor TBX3
• UniProt Protein Name: T-box transcription factor TBX3
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX3
• UniProt Synonym Gene Names: T-box protein 3

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence.