Androgen Receptor ELISA Kit | AR elisa kit

Androgen Receptor (Phospho-Ser650) TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502130

• Gene Names: AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• Reactivity: Human, Mouse, Rat
• Synonyms: Androgen Receptor; Androgen Receptor (Phospho-Ser650) TFact DNA-Binding ELISA Kit; ANDR; Androgen receptor; DHTR; Dihydrotestosterone receptor; NR3C4; AR elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 920

• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact Androgen Receptor (Phospho-Ser650) DNA-Binding ELISA detects active Androgen Receptor (Phospho-Ser650) in MCF7 Nuclear Extract. MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

Related Product Information for AR elisa kit

Principle of the Assay: The TFactTM DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample. Currently, the most common methods to detect transcription factor binding to DNA elements and motifs are electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation, western blotting, and expression of fused target and reporter genes. These methods are often time consuming, complicated, and make it difficult to achieve satisfactory results. Assay Biotech TFact™ DNA-Binding ELISA Kits can significantly reduce the necessary runtime to within one day and eliminate the need for harmful radioactive labeling while maintaining high sensitivity and signal-to-noise ratio. In the past, it was strenuous and inefficient to perform high-throughput screening for hundreds of different samples or transcription factors. Today, our revolutionary TFact™ DNA-Binding ELISA Kits can eliminate these challenges and help expedite the journey from research to publication or product.

NCBI and Uniprot Product Information

• NCBI GI #: 21322252
• NCBI GeneID: 367
• NCBI Accession #: NP_000035.2
• NCBI GenBank Nucleotide #: NM_000044.4
• UniProt Accession #: P10275; Q9UD95; A0A0B4J1T2; A2RUN2; B1AKD7; C0JKD3; C0JKD4; E7EVX6
• Molecular Weight: 67,659 Da
• NCBI Official Full Name: androgen receptor isoform 1
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: AR
• NCBI Official Synonym Symbols: KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• NCBI Protein Information: androgen receptor
• UniProt Protein Name: Androgen receptor
• Protein Family: Allatostatin
• UniProt Gene Name: AR
• UniProt Synonym Gene Names: DHTR; NR3C4

NCBI Description

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Uniprot Description

AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nuclear speck; nucleoplasm; nucleus; plasma membrane; protein complex

Molecular Function: androgen binding; androgen receptor activity; ATPase binding; beta-catenin binding; chromatin binding; DNA binding; DNA binding transcription factor activity; enzyme binding; ligand-dependent nuclear receptor activity; POU domain binding; protein binding; protein dimerization activity; receptor binding; steroid binding; transcription factor binding; transcription regulatory region DNA binding; zinc ion binding

Biological Process: androgen receptor signaling pathway; animal organ formation; cell growth; cell proliferation; cell-cell signaling; cellular response to steroid hormone stimulus; cellular response to testosterone stimulus; epithelial cell morphogenesis; in utero embryonic development; intracellular receptor signaling pathway; Leydig cell differentiation; male genitalia morphogenesis; male somatic sex determination; multicellular organism growth; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of extrinsic apoptotic signaling pathway; negative regulation of integrin biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of gene expression; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of integrin biosynthetic process; positive regulation of intracellular estrogen receptor signaling pathway; positive regulation of MAPK cascade; positive regulation of NF-kappaB transcription factor activity; positive regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription, DNA-templated; prostate gland development; protein deubiquitination; protein oligomerization; regulation of catalytic activity; regulation of developmental growth; regulation of systemic arterial blood pressure; seminiferous tubule development; sex differentiation; signal transduction; single fertilization; spermatogenesis; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transport

Disease: Androgen Insensitivity Syndrome; Androgen Insensitivity, Partial; Hypospadias 1, X-linked; Prostate Cancer; Spinal And Bulbar Muscular Atrophy, X-linked 1