Human, Mouse CDX2 ELISA Kit | CDX2 elisa kit

CDX2 TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502146

• Gene Names: CDX2; CDX3; CDX-3; CDX2/AS
• Reactivity: Human, Mouse
• Synonyms: CDX2; CDX2 TFact DNA-Binding ELISA Kit; caudal type homeo box transcription factor 2; Caudal-type homeobox protein 2; CDX-3; CDX3; Homeobox protein CDX-2; CDX2 elisa kit

• Reactivity: Human, Mouse
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 313

• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact CDX2 DNA-Binding ELISA detects active CDX2 in HEK293 Nuclear Extract. The HEK293 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

Related Product Information for CDX2 elisa kit

Principle of the Assay: The TFactTM DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample. Currently, the most common methods to detect transcription factor binding to DNA elements and motifs are electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation, western blotting, and expression of fused target and reporter genes. These methods are often time consuming, complicated, and make it difficult to achieve satisfactory results. The TFact™ DNA-Binding ELISA Kits can significantly reduce the necessary runtime to within one day and eliminate the need for harmful radioactive labeling while maintaining high sensitivity and signal-to-noise ratio. In the past, it was strenuous and inefficient to perform high-throughput screening for hundreds of different samples or transcription factors. Today, our revolutionary TFact™ DNA-Binding ELISA Kits can eliminate these challenges and help expedite the journey from research to publication or product.

NCBI and Uniprot Product Information

• NCBI GI #: 372624392
• NCBI GeneID: 1045
• NCBI Accession #: NP_001256.3
• NCBI GenBank Nucleotide #: NM_001265.5
• UniProt Accession #: Q99626; O00503; Q5VTU7; Q969L8; Q9UD92
• Molecular Weight: 33,520 Da
• NCBI Official Full Name: homeobox protein CDX-2 isoform 1
• NCBI Official Synonym Full Names: caudal type homeobox 2
• NCBI Official Symbol: CDX2
• NCBI Official Synonym Symbols: CDX3; CDX-3; CDX2/AS
• NCBI Protein Information: homeobox protein CDX-2
• UniProt Protein Name: Homeobox protein CDX-2
• Protein Family: Homeobox protein
• UniProt Gene Name: CDX2
• UniProt Synonym Gene Names: CDX3

NCBI Description

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]

Uniprot Description

CDX2: Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Belongs to the Caudal homeobox family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q12.2

Cellular Component: condensed nuclear chromosome; nucleoplasm; nucleus; protein complex; transcriptional repressor complex

Molecular Function: DNA binding transcription factor activity; transcription corepressor activity

Biological Process: anterior/posterior axis specification; blood vessel development; cell differentiation; endosome to lysosome transport; establishment or maintenance of epithelial cell apical/basal polarity; negative regulation of transcription from RNA polymerase II promoter; organ morphogenesis; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of transcription, DNA-templated; regulation of somitogenesis; somatic stem cell maintenance; transcription from RNA polymerase II promoter; trophectodermal cell differentiation