STAT1 elisa kit

STAT1 (Phospho-Tyr701) TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502282

• Gene Names: STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• Reactivity: Human, Mouse, Rat
• Synonyms: STAT1; STAT1 (Phospho-Tyr701) TFact DNA-Binding ELISA Kit; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 750

• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact STAT1 (Phospho-Tyr701) DNA-Binding ELISA detects active STAT1 (Phospho-Tyr701) in HepG2 Nuclear Extract. The HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)

Related Product Information for STAT1 elisa kit

Principle of the Assay: The TFactTM DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample. Currently, the most common methods to detect transcription factor binding to DNA elements and motifs are electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation, western blotting, and expression of fused target and reporter genes. These methods are often time consuming, complicated, and make it difficult to achieve satisfactory results. TFact™ DNA-Binding ELISA Kits can significantly reduce the necessary runtime to within one day and eliminate the need for harmful radioactive labeling while maintaining high sensitivity and signal-to-noise ratio. In the past, it was strenuous and inefficient to perform high-throughput screening for hundreds of different samples or transcription factors. Today, our revolutionary TFact™ DNA-Binding ELISA Kits can eliminate these challenges and help expedite the journey from research to publication or product.

NCBI and Uniprot Product Information

• NCBI GI #: 6274552
• NCBI GeneID: 6772
• NCBI Accession #: NP_009330.1
• NCBI GenBank Nucleotide #: NM_007315.3
• UniProt Accession #: P42224; Q53S88; Q53XW4; Q68D00; Q9UDL5; A8K989; B2RCA0; D2KFR8; D3DPI7
• Molecular Weight: 83,043 Da
• NCBI Official Full Name: signal transducer and activator of transcription 1-alpha/beta isoform alpha
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1
• NCBI Official Symbol: STAT1
• NCBI Official Synonym Symbols: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• NCBI Protein Information: signal transducer and activator of transcription 1-alpha/beta
• UniProt Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT1

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: axon; cytoplasm; cytosol; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: cadherin binding; CCR5 chemokine receptor binding; DNA binding transcription factor activity; double-stranded DNA binding; enzyme binding; histone acetyltransferase binding; histone binding; identical protein binding; nuclear hormone receptor binding; promoter-specific chromatin binding; protein binding; protein homodimerization activity; protein phosphatase 2A binding; signal transducer activity; tumor necrosis factor receptor binding; ubiquitin-like protein ligase binding

Biological Process: apoptosis; blood circulation; cellular response to insulin stimulus; cellular response to interferon-gamma; cellular response to organic cyclic compound; defense response to virus; endothelial cell migration; JAK-STAT cascade; macrophage derived foam cell differentiation; metanephric mesenchymal cell differentiation; metanephric mesenchymal cell proliferation involved in metanephros development; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; negative regulation of metanephric nephron tubule epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of viral protein levels in host cell; positive regulation of defense response to virus by host; positive regulation of erythrocyte differentiation; positive regulation of interferon-alpha production; positive regulation of mesenchymal cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of apoptosis; response to cAMP; response to cytokine; response to drug; response to hydrogen peroxide; response to mechanical stimulus; response to nutrient; response to peptide hormone; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway; viral process

Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c