C/EBP-beta elisa kit

C/EBP-beta (Phospho-Thr235/188) TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502279

• Gene Names: CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
• Reactivity: Human, Mouse, Rat
• Synonyms: C/EBP-beta; C/EBP-beta (Phospho-Thr235/188) TFact DNA-Binding ELISA Kit; AGP/EBP; C/EBP beta; C/EBP-related protein 2; CCAAT/enhancer binding protein beta; CEBPB; CRP2; IL-6DBP; Interleukin-6- dependent binding protein; LAP; Liver-enriched transcriptional activator; Nuclear factor NF-IL6; SF-B; SFB; Silencer factor B; TCF5; Transcription factor 5; C/EBP-beta elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 322

• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact C/EBP-beta (Phospho-Thr235/188) DNA-Binding ELISA Kit detects active C/EBP-beta (Phospho-Thr235/188) in HepG2 Nuclear Extract. The HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. )

Related Product Information for C/EBP-beta elisa kit

Principle of the Assay: The TFactTM DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample. Currently, the most common methods to detect transcription factor binding to DNA elements and motifs are electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation, western blotting, and expression of fused target and reporter genes. These methods are often time consuming, complicated, and make it difficult to achieve satisfactory results. Assay Biotech TFact™ DNA-Binding ELISA Kits can significantly reduce the necessary runtime to within one day and eliminate the need for harmful radioactive labeling while maintaining high sensitivity and signal-to-noise ratio. In the past, it was strenuous and inefficient to perform high-throughput screening for hundreds of different samples or transcription factors. Today, our revolutionary TFact™ DNA-Binding ELISA Kits can eliminate these challenges and help expedite the journey from research to publication or product.

NCBI and Uniprot Product Information

• NCBI GI #: 551895085
• NCBI GeneID: 1051
• NCBI Accession #: NP_001272807.1
• NCBI GenBank Nucleotide #: NM_001285878.1
• UniProt Accession #: P17676; Q96IH2; Q9H4Z5; A8K671
• Molecular Weight: 15,938 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform b
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: TCF5; IL6DBP; NF-IL6; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: TCF-5

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Uniprot Description

Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:9374525, PubMed:12048245, PubMed:18647749). Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:20829347). Essential for female reproduction because of a critical role in ovarian follicle development (). Restricts osteoclastogenesis ().