Western Blot (WB)
(Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (MM0271) at 1:1000.)
Mouse anti-Human Mitofilin Monoclonal Antibody | anti-IMMT antibody
Mitofilin Antibody
Gene Names
IMMT; HMP; P87; P89; PIG4; PIG52; MINOS2; P87/89
Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry, Immunoprecipitation
Purity
Protein G chromatography
Synonyms
Mitofilin; Monoclonal Antibody; Mitofilin Antibody; Mitofiin M027; anti-IMMT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M027
Specificity
Clone M027 detects a 90kDa* band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. In addition, mass spectrometry analysis of immunoprecipitates usingmM0271 in human A431 cell lysates confirms this antibody only detects Mitofilin protein. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-IMMT antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
ELISA: 1:2000
ICC: 1:100
IP: 1:100
WB: 1:1000
ICC: 1:100
IP: 1:100
WB: 1:1000
Immunogen
Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (MM0271) at 1:1000.)
Western Blot (WB)
(Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (MM0271) at 1:1000.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight 594.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight 594.)
Related Product Information for anti-IMMT antibody
Mitofilin is an important organizer of mitochondrial architecture. The mitofilin sequence encodes a polypeptide with a central alpha-helical region with predicted coiled coil domains flanked by globular amino and carboxy termini. There are four isoforms of mitofilin, and 90/91kDa mitofilin forms have been observed in western blots. Mitofilin is located in the inner mitochondrial membrane and interacts with several protein complexes of the outer membrane, thereby generating contact sites between the two membrane systems of mitochondria. These mitofilin-containing hetero-oligomeric protein complexes form the mitochondrial inner membrane organizing system (MINOS). MINOS integrity is required for the structural maintenance of the inner mitochondrial membrane. This mitochondrial region contains cristae membranes that form large tubular invaginations that protrude into the mitochondrial matrix. These cristae membranes contain the enzyme complexes of the oxidative phosphorylation machinery. MINOS deficiency causes loss of crista junction structures and the detachment of cristae from the inner boundary membrane.
References
Zerbes RM, et al. (2012) Biol Chem. 393(11):1247.
Odgren PR, et al. (1996) J Cell Sci. 109(9):2253.
Odgren PR, et al. (1996) J Cell Sci. 109(9):2253.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
758
NCBI Official Full Name
MICOS complex subunit MIC60
NCBI Official Synonym Full Names
inner membrane protein, mitochondrial
NCBI Official Symbol
IMMT
NCBI Official Synonym Symbols
HMP; P87; P89; PIG4; PIG52; MINOS2; P87/89
NCBI Protein Information
MICOS complex subunit MIC60; mitofilin; motor protein; proliferation-inducing gene 4; mitochondrial inner membrane protein; cell proliferation-inducing protein 52; cell proliferation-inducing gene 4/52 protein; mitochondrial inner membrane organizing syst
UniProt Protein Name
MICOS complex subunit MIC60
UniProt Gene Name
IMMT
UniProt Synonym Gene Names
HMP; MIC60; MINOS2
UniProt Entry Name
MIC60_HUMAN
Uniprot Description
IMMT: 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2p11.2|2
Cellular Component: mitochondrion; membrane; mitochondrial inner membrane; integral to membrane
Molecular Function: protein binding
Biological Process: mitochondrial calcium ion homeostasis; response to cold
Research Articles on IMMT
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Product Notes
The IMMT immt (Catalog #AAA474441) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mitofilin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mitofilin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunoprecipitation (IP). ELISA: 1:2000 ICC: 1:100 IP: 1:100 WB: 1:1000. Researchers should empirically determine the suitability of the IMMT immt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitofilin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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