CREB elisa kit

CREB (Phospho-Ser133) TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502143

• Gene Names: CREB1; CREB; CREB-1
• Reactivity: Human, Mouse, Rat
• Synonyms: CREB; CREB (Phospho-Ser133) TFact DNA-Binding ELISA Kit; cAMP responsive element binding protein 1; cAMP-response element binding protein; CREB-1; CREB1; CREB elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 327

• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact CREB DNA-Binding ELISA kits detect active CREB in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.)

Related Product Information for CREB elisa kit

Principle of the Assay: The TFactTM DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample. Currently, the most common methods to detect transcription factor binding to DNA elements and motifs are electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation, western blotting, and expression of fused target and reporter genes. These methods are often time consuming, complicated, and make it difficult to achieve satisfactory results. Assay Biotech TFact™ DNA-Binding ELISA Kits can significantly reduce the necessary runtime to within one day and eliminate the need for harmful radioactive labeling while maintaining high sensitivity and signal-to-noise ratio. In the past, it was strenuous and inefficient to perform high-throughput screening for hundreds of different samples or transcription factors. Today, our revolutionary TFact™ DNA-Binding ELISA Kits can eliminate these challenges and help expedite the journey from research to publication or product.

NCBI and Uniprot Product Information

• NCBI GI #: 4758054
• NCBI GeneID: 1385
• NCBI Accession #: NP_004370.1
• NCBI GenBank Nucleotide #: NM_004379.4
• UniProt Accession #: P16220; P21934; Q6V963; Q9UMA7
• Molecular Weight: 25,445 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform A
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB; CREB-1
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: CREB-binding protein
• UniProt Gene Name: CREB1
• UniProt Synonym Gene Names: CREB-1; cAMP-responsive element-binding protein 1

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: ATF4-CREB1 transcription factor complex; axon; mitochondrial matrix; nuclear euchromatin; nucleoplasm; nucleus

Molecular Function: DNA binding transcription factor activity; enzyme binding; histone acetyltransferase binding; Hsp70 protein binding; identical protein binding; protein binding; transcription cofactor activity; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding

Biological Process: aging; axon guidance; cellular response to fatty acid; cellular response to insulin-like growth factor stimulus; cellular response to zinc ion; chemotaxis to arachidonic acid; circadian rhythm; lactation; memory; negative regulation of neuron death; negative regulation of transcription by competitive promoter binding; pituitary gland development; positive regulation of apoptosis; positive regulation of cardiac muscle tissue development; positive regulation of fat cell differentiation; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; positive regulation of long-term synaptic potentiation; positive regulation of multicellular organism growth; positive regulation of osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of transcriptional preinitiation complex assembly; positive regulation of transforming growth factor beta3 production; protein amino acid phosphorylation; protein kinase B signaling; protein stabilization; regulation of cell size; regulation of circadian rhythm; regulation of fibroblast proliferation; response to activity; response to drug; response to glucagon; response to hypoxia; response to L-glutamate; response to nicotine; response to organic substance; secretory granule organization and biogenesis; signal transduction; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; viral process; visual learning

Disease: Histiocytoma, Angiomatoid Fibrous