Testing Data
(The TFact HIF-1alpha DNA-Binding ELISA detects active HIF-1alpha in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)
HIF-1alpha elisa kit
HIF-1alpha TFact DNA-Binding ELISA Kit
Testing Data
(The TFact HIF-1alpha DNA-Binding ELISA detects active HIF-1alpha in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)
Testing Data
(The TFact HIF-1alpha DNA-Binding ELISA detects active HIF-1alpha in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Uniprot Description
HIF1A: a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia- mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome- mediated degradation. Interacts (via N-terminus) with USP19. Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF, FGF2, IGF2, TGFB1, HGF, TNF, IL1B, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia. Repressed by HIPK2 and LIMD1. Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 14q23.2
Cellular Component: axon cytoplasm; cytoplasm; cytosol; nuclear body; nuclear speck; nucleoplasm; nucleus; protein complex; RNA polymerase II transcription factor complex; transcription factor complex
Molecular Function: DNA binding transcription factor activity; enzyme binding; histone acetyltransferase binding; histone deacetylase binding; Hsp90 protein binding; nuclear hormone receptor binding; p53 binding; protein binding; protein domain specific binding; protein heterodimerization activity; protein kinase binding; sequence-specific DNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; transcription factor binding; ubiquitin protein ligase binding
Biological Process: angiogenesis; axonal transport of mitochondrion; B-1 B cell homeostasis; cardiac ventricle morphogenesis; cartilage development; cellular iron ion homeostasis; cellular response to hypoxia; cellular response to interleukin-1; cerebral cortex development; collagen metabolic process; connective tissue replacement during inflammatory response; digestive tract morphogenesis; dopaminergic neuron differentiation; elastin metabolic process; embryonic hemopoiesis; embryonic placenta development; epithelial to mesenchymal transition; glucose homeostasis; heart looping; hemoglobin biosynthetic process; lactate metabolic process; lactation; mRNA transcription from RNA polymerase II promoter; muscle maintenance; negative regulation of bone mineralization; negative regulation of gene expression; negative regulation of growth; negative regulation of mesenchymal cell apoptotic process; negative regulation of reactive oxygen species metabolic process; negative regulation of TOR signaling; neural crest cell migration; neural fold elevation formation; outflow tract morphogenesis; oxygen homeostasis; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of chemokine production; positive regulation of chemokine-mediated signaling pathway; positive regulation of endothelial cell proliferation; positive regulation of epithelial cell migration; positive regulation of erythrocyte differentiation; positive regulation of gene expression; positive regulation of glycolysis; positive regulation of hormone biosynthetic process; positive regulation of macroautophagy; positive regulation of neuroblast proliferation; positive regulation of nitric-oxide synthase activity; positive regulation of receptor biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of vascular endothelial growth factor production; positive regulation of vascular endothelial growth factor receptor signaling pathway; post-translational protein modification; protein deubiquitination; protein ubiquitination; regulation of gene expression; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; regulation of transcription, DNA-templated; regulation of transforming growth factor-beta2 production; response to hypoxia; response to iron ion; response to muscle activity; signal transduction; transcription from RNA polymerase II promoter; vascular endothelial growth factor production; visual learning
Research Articles on HIF-1alpha
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Product Notes
The Human, Mouse, Rat HIF-1alpha hif1a (Catalog #AAA9502190) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9502190 ELISA Kit recognizes Human, Mouse, Rat HIF-1alpha. It is sometimes possible for the material contained within the vial of "HIF-1alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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