Typical Testing Data/Standard Curve (for reference only)
()
Human Programmed Death Ligand 1 (PD-L1/B7-H1/CD274) ELISA Kit | PD-L1/B7-H1/CD274 elisa kit
Human Programmed Death Ligand 1 ELISA Kit (PD-L1/B7-H1/CD274)
Reactivity
Human
Synonyms
Programmed Death Ligand 1 (PD-L1/B7-H1/CD274); Human Programmed Death Ligand 1 ELISA Kit (PD-L1/B7-H1/CD274); PD-L1/B7-H1/CD274 elisa kit
Reactivity
Human
Specificity
This assay recognizes both recombinant and natural human PD-L1. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
Samples
Cell culture supernates, serum and plasma
Assay Type
Quantitative Sandwich
Sensitivity
< 0.056ng/mL
Intra-assay Precision
Intra-plate Precision 3 samples with low, middle and high level PD-L1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-plate Precision 3 samples with low, middle and high level PD-L1 were tested on 3 different plates, 8 replicates in each plate. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Related Product Information for PD-L1/B7-H1/CD274 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PD-L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PD-L1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for PD-L1 is added to the wells and binds to the combination of capture antibody PD-L1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of PD-L1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven PD-L1 standard dilutions and PD-L1 sample concentration determined.
Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of human PD-L1 concentrations in cell culture supernates, serum and plasma.
Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of human PD-L1 concentrations in cell culture supernates, serum and plasma.
Similar Products
Product Notes
The Human PD-L1/B7-H1/CD274 (Catalog #AAA9141977) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9141977 ELISA Kit recognizes Human PD-L1/B7-H1/CD274. It is sometimes possible for the material contained within the vial of "Programmed Death Ligand 1 (PD-L1/B7-H1/CD274), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
