Bovine Fibrinogen gamma-B chain ELISA Kit | FGG elisa kit

Bovine Fibrinogen gamma-B chain ELISA Kit

Cat. Num. AAA288011

• Reactivity: Bovine
• Synonyms: Fibrinogen gamma-B chain; Bovine Fibrinogen gamma-B chain ELISA Kit; FGG elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural bovine Gamma'. No significant cross-reactivity or interference was observed.
• Sequence Length: 444

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 7.80 ng/ml -500 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine Gamma' concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGG elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma'. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Gamma' and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gamma', biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Gamma' in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27806893
• NCBI GeneID: 280792
• NCBI Accession #: NP_776336.1
• NCBI GenBank Nucleotide #: NM_173911.2
• UniProt Accession #: P12799
• Molecular Weight: 50,244 Da
• NCBI Official Full Name: fibrinogen gamma-B chain
• NCBI Official Symbol: FGG
• NCBI Protein Information: fibrinogen gamma-B chain
• UniProt Protein Name: Fibrinogen gamma-B chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGG
• UniProt Synonym Gene Names: Gamma'
• UniProt Entry Name: FIBG_BOVIN

Uniprot Description

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.