Human MANF ELISA Kit | MANF elisa kit

Human MANF PicoKine ELISA Kit

Cat. Num. AAA1751657

• Gene Names: MANF; ARP; ARMET
• Reactivity: Human
• Synonyms: MANF; Human MANF PicoKine ELISA Kit; Human MANF; MANF elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MADCAM1
• Sequence Length: 182

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MANF elisa kit

Principle of the Assay: The Picokine Human MADCAM1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MADCAM1 with a 96-well strip plate that is pre-coated with antibody specific for MADCAM1. The detection antibody is a biotinylated antibody specific for MADCAM1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human MADCAM1 with immunogen: Expression system for standard: NSO, Immunogen sequence: Q19-Q317. The kit is analytically validated with ready to use reagents. To measure Human MADCAM1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human MADCAM1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MADCAM1 in the sample!!Background/Introduction: MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4/beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.

NCBI and Uniprot Product Information

• NCBI GI #: 1478051060
• NCBI GeneID: 7873
• NCBI Accession #: NP_006001.5
• NCBI GenBank Nucleotide #: NM_006010.6
• UniProt Accession #: P55145
• NCBI Official Full Name: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Synonym Full Names: mesencephalic astrocyte derived neurotrophic factor
• NCBI Official Symbol: MANF
• NCBI Official Synonym Symbols: ARP; ARMET
• NCBI Protein Information: mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: MANF/CDNF-like protein

NCBI Description

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]