Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human T-lymphocyte surface antigen Ly-9 (LY9) Standard Curve.)
Human T-Lymphocyte Surface Antigen Ly-9 (LY9) ELISA Kit | LY9 elisa kit
Human T-Lymphocyte Surface Antigen Ly-9 (LY9) ELISA Kit
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Reactivity
Human
Synonyms
T-Lymphocyte Surface Antigen Ly-9 (LY9); Human T-Lymphocyte Surface Antigen Ly-9 (LY9) ELISA Kit; LY9; RP11-312J18.1; CD229; SLAMF3; hly9; mLY9; T-lymphocyte surface antigen Ly-9; cell-surface molecule Ly-9; LY9 elisa kit
Reactivity
Human
Specificity
Human T-lymphocyte surface antigen Ly-9 (LY9) ELISA Kit has high sensitivity and excellent specificity for detection of Human LY9. No significant cross-reactivity or interference between Human LY9 and analogues was observed.
Sequence Length
193
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human T-lymphocyte surface antigen Ly-9 (LY9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human T-lymphocyte surface antigen Ly-9 (LY9) Standard Curve.)
Related Product Information for LY9 elisa kit
Description: This Human T-lymphocyte surface antigen Ly-9 (LY9) ELISA Kit employs a two-site sandwich ELISA to quantitate LY9 in samples. An antibody specific for LY9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LY9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lymphocyte antigen 9, also known as LY9, has also recently been designated CD229. LY9 has been shown to interact with SH2D1A.By sequence analysis of cDNA clones, Sandrin et al. (1992) demonstrated that the mouse lymphocyte surface antigen Ly9 is a novel member of a subgroup of the immunoglobulin superfamily, together with the antigens CD2, BCM1 (CD48), and LFA3 (CD58). Kingsmore et al. (1995) noted that, though the function of Ly9 was unknown, other subgroup members are involved in adhesion reactions between T lymphocytes and accessory cells. CD2, CD48, and CD58 are also distinguished by homophilic interactions with one another; thus, human CD48 and CD58 are both ligands for CD2.
Background: Lymphocyte antigen 9, also known as LY9, has also recently been designated CD229. LY9 has been shown to interact with SH2D1A.By sequence analysis of cDNA clones, Sandrin et al. (1992) demonstrated that the mouse lymphocyte surface antigen Ly9 is a novel member of a subgroup of the immunoglobulin superfamily, together with the antigens CD2, BCM1 (CD48), and LFA3 (CD58). Kingsmore et al. (1995) noted that, though the function of Ly9 was unknown, other subgroup members are involved in adhesion reactions between T lymphocytes and accessory cells. CD2, CD48, and CD58 are also distinguished by homophilic interactions with one another; thus, human CD48 and CD58 are both ligands for CD2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
T-lymphocyte surface antigen Ly-9 isoform b
NCBI Official Synonym Full Names
lymphocyte antigen 9
NCBI Official Symbol
LY9
NCBI Official Synonym Symbols
hly9; mLY9; CD229; SLAMF3
NCBI Protein Information
T-lymphocyte surface antigen Ly-9
UniProt Protein Name
T-lymphocyte surface antigen Ly-9
Protein Family
UniProt Gene Name
LY9
UniProt Synonym Gene Names
SLAMF3
NCBI Description
LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM, Mar 2008]
Uniprot Description
CD229: May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: plasma membrane
Biological Process: positive regulation of interleukin-17 production
Research Articles on LY9
Similar Products
Product Notes
The Human LY9 ly9 (Catalog #AAA9716034) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716034 ELISA Kit recognizes Human LY9. It is sometimes possible for the material contained within the vial of "T-Lymphocyte Surface Antigen Ly-9 (LY9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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