Typical Testing Data/Standard Curve (for reference only)
Rat BDNF ELISA Kit | BDNF elisa kit
Rat BDNF PicoKine ELISA Kit
Reactivity
Rat
Synonyms
BDNF; Rat BDNF PicoKine ELISA Kit; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; Abrineurin; ANON2; Brain derived neurotrophic factor; BULN2; MGC34632; Neurotrophin; BDNF elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Agrn
Sequence Length
249
Samples
Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BDNF elisa kit
Principle of the Assay: The Picokine Rat Agrn Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Agrn with a 96-well strip plate that is pre-coated with antibody specific for Agrn. The detection antibody is a biotinylated antibody specific for Agrn. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Agrn with immunogen: Expression system for standard: sf21, Immunogen sequence: A1153-P1959. The kit is analytically validated with ready to use reagents. To measure Rat Agrn, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Agrn in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Agrn in the sample!!Background/Introduction: Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRN gene.This protein has nine domains homologous to protease inhibitors.It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cellmatrix interactions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,109 Da
NCBI Official Full Name
Brain-derived neurotrophic factor
NCBI Official Synonym Full Names
brain-derived neurotrophic factor
NCBI Official Symbol
Bdnf
NCBI Protein Information
brain-derived neurotrophic factor; brain derived neurotrophic factor; brain derived neurothrophic factor
UniProt Protein Name
Brain-derived neurotrophic factor
Protein Family
UniProt Gene Name
Bdnf
UniProt Synonym Gene Names
BDNF
UniProt Entry Name
BDNF_RAT
NCBI Description
plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006]
Uniprot Description
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS ().
Research Articles on BDNF
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Product Notes
The Rat BDNF bdnf (Catalog #AAA175935) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175935 ELISA Kit recognizes Rat BDNF. It is sometimes possible for the material contained within the vial of "BDNF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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